Detailed information of TA system TA241744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 11565..12292
• Replicon: plasmid p21080237_1
• Accession: NZ_CP095241
• Organism: Klebsiella pneumoniae strain 21080237

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: MU300_RS26545
• Protein ID: WP_011251285.1
• Coordinates: 11981..12292 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MU300_RS26540
• Protein ID: WP_011251286.1
• Coordinates: 11565..11984 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU300_RS26510 (MU300_26505)	6733..7203	-	471	WP_048333570.1	hypothetical protein	-
MU300_RS26515 (MU300_26510)	7516..8151	-	636	WP_223171879.1	hypothetical protein	-
MU300_RS26520 (MU300_26515)	8570..9190	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
MU300_RS26525 (MU300_26520)	9211..9999	+	789	WP_040217257.1	hypothetical protein	-
MU300_RS26530 (MU300_26525)	10013..10378	+	366	WP_048333448.1	hypothetical protein	-
MU300_RS26535 (MU300_26530)	10450..11418	-	969	WP_074428168.1	IS5 family transposase	-
MU300_RS26540 (MU300_26535)	11565..11984	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
MU300_RS26545 (MU300_26540)	11981..12292	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
MU300_RS26550 (MU300_26545)	12497..12934	-	438	Protein_14	DDE-type integrase/transposase/recombinase	-
MU300_RS26555 (MU300_26550)	13069..13766	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
MU300_RS26560 (MU300_26555)	15152..15802	-	651	WP_068893702.1	DUF1173 family protein	-
MU300_RS26565 (MU300_26560)	15835..16101	-	267	WP_223175074.1	DUF1173 family protein	-
MU300_RS26570 (MU300_26565)	16279..16773	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..188001	188001
	-	inside	IScluster/Tn	-	-	117..18959	18842

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T241744 WP_011251285.1 NZ_CP095241:c12292-11981 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T241744 NZ_OU015340:c2396425-2396138 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT241744 WP_011251286.1 NZ_CP095241:c11984-11565 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT241744 NZ_OU015340:c2396151-2395849 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1