Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 2580238..2580824 Replicon chromosome
Accession NZ_CP095240
Organism Klebsiella pneumoniae strain 21080237

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag MU300_RS12630 Protein ID WP_002920800.1
Coordinates 2580456..2580824 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag MU300_RS12625 Protein ID WP_002920802.1
Coordinates 2580238..2580459 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU300_RS12605 (MU300_12600) 2576395..2577321 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MU300_RS12610 (MU300_12605) 2577318..2578595 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
MU300_RS12615 (MU300_12610) 2578592..2579359 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MU300_RS12620 (MU300_12615) 2579361..2580074 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MU300_RS12625 (MU300_12620) 2580238..2580459 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MU300_RS12630 (MU300_12625) 2580456..2580824 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MU300_RS12635 (MU300_12630) 2581097..2582413 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MU300_RS12640 (MU300_12635) 2582520..2583407 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MU300_RS12645 (MU300_12640) 2583404..2584249 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MU300_RS12650 (MU300_12645) 2584251..2585321 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2577318..2586058 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T241736 WP_002920800.1 NZ_CP095240:2580456-2580824 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T241736 NZ_OU015323:3618429-3618704 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT241736 WP_002920802.1 NZ_CP095240:2580238-2580459 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT241736 NZ_OU015323:3618166-3618426 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References