Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 14827..15554 Replicon plasmid p21072329_2
Accession NZ_CP095236
Organism Klebsiella pneumoniae strain 21072329

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag MU289_RS28115 Protein ID WP_004118600.1
Coordinates 14827..15138 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag MU289_RS28120 Protein ID WP_000990392.1
Coordinates 15135..15554 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU289_RS28090 (10544) 10544..11266 + 723 Protein_14 D-lactate dehydrogenase -
MU289_RS28095 (11341) 11341..12060 + 720 WP_001102107.1 (Fe-S)-binding protein -
MU289_RS28100 (12071) 12071..13498 + 1428 WP_000023895.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
MU289_RS28105 (13491) 13491..14186 + 696 WP_004118595.1 lactate utilization protein C -
MU289_RS28110 (14236) 14236..14616 - 381 Protein_18 gluconate permease -
MU289_RS28115 (14827) 14827..15138 + 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
MU289_RS28120 (15135) 15135..15554 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
MU289_RS28125 (15591) 15591..16793 - 1203 WP_003031541.1 hypothetical protein -
MU289_RS28130 (16786) 16786..17115 - 330 WP_004118613.1 hypothetical protein -
MU289_RS28135 (17112) 17112..17771 - 660 WP_000078540.1 hypothetical protein -
MU289_RS28140 (18195) 18195..18503 - 309 WP_020324596.1 Ref family recombination enhancement nuclease -
MU289_RS28145 (18671) 18671..18811 - 141 WP_004118614.1 hypothetical protein -
MU289_RS28150 (19104) 19104..19457 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
MU289_RS28155 (19505) 19505..19867 + 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId - 1..58972 58972


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T241722 WP_004118600.1 NZ_CP095236:14827-15138 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T241722 NZ_OD940440:2718266-2718409 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT241722 WP_000990392.1 NZ_CP095236:15135-15554 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT241722 NZ_OD940440:c2718171-2717973 [Enterococcus faecalis]
TTTTTAGGTAATTGTGCTATAATGGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References