Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2706408..2707054 Replicon chromosome
Accession NZ_CP095234
Organism Klebsiella pneumoniae strain 21072329

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag MU289_RS13015 Protein ID WP_002920560.1
Coordinates 2706408..2706755 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag MU289_RS13020 Protein ID WP_002920557.1
Coordinates 2706755..2707054 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU289_RS13005 (MU289_13005) 2702334..2703767 + 1434 WP_002920564.1 glycogen synthase GlgA -
MU289_RS13010 (MU289_13010) 2703785..2706232 + 2448 WP_002920561.1 glycogen phosphorylase -
MU289_RS13015 (MU289_13015) 2706408..2706755 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MU289_RS13020 (MU289_13020) 2706755..2707054 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
MU289_RS13025 (MU289_13025) 2707117..2708625 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
MU289_RS13030 (MU289_13030) 2708830..2709159 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
MU289_RS13035 (MU289_13035) 2709210..2710040 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
MU289_RS13040 (MU289_13040) 2710090..2710848 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T241707 WP_002920560.1 NZ_CP095234:2706408-2706755 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T241707 NZ_OD940437:622478-622594 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT241707 WP_002920557.1 NZ_CP095234:2706755-2707054 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT241707 NZ_OD940437:c622710-622505 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References