Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/VapI(antitoxin)
Location 1123648..1125098 Replicon chromosome
Accession NC_012988
Organism Methylobacterium extorquens DM4 (Methylobacterium)

Toxin (Protein)


Gene name higB Uniprot ID C7CEU6
Locus tag METD_RS05320 Protein ID WP_003602533.1
Coordinates 1123648..1123986 (+) Length 113 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag METD_RS05325 Protein ID WP_003602534.1
Coordinates 1124004..1125098 (+) Length 365 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
METD_RS30440 1119914..1120902 - 989 Protein_1066 Cj0069 family protein -
METD_RS05305 1121255..1121683 - 429 WP_003602529.1 hypothetical protein -
METD_RS05310 1121808..1123142 - 1335 WP_015821465.1 MFS transporter -
METD_RS05315 1123150..1123413 - 264 WP_003602532.1 metal-sensing transcriptional repressor -
METD_RS05320 1123648..1123986 + 339 WP_003602533.1 hypothetical protein Toxin
METD_RS05325 1124004..1125098 + 1095 WP_003602534.1 HigA family addiction module antidote protein Antitoxin
METD_RS05330 1125231..1125527 + 297 WP_003602536.1 hypothetical protein -
METD_RS31185 1125851..1126006 - 156 Protein_1073 IS481 family transposase -
METD_RS05340 1125987..1126418 - 432 WP_003602540.1 hypothetical protein -
METD_RS05345 1126587..1126952 - 366 WP_003602542.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(16-68)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12576.35 Da        Isoelectric Point: 9.0704

>T24169 WP_003602533.1 NC_012988:1123648-1123986 [Methylorubrum extorquens AM1]
MDLTVRGKRLGKQLTDPREMDRAFGNRARHIRLRLGVLRQAKCLSDVPTGSPDYRHMLTGDRKGQFSVTIRDNWRIVFVP
DHDPVPSLPDGGVDVASITAVEIVEVVDYHGN

Download         Length: 339 bp

>T24169 NC_012988:1123648-1123986 [Methylorubrum extorquens AM1]
ATGGATCTCACCGTCCGAGGTAAGCGGCTGGGCAAGCAGCTTACCGACCCGAGGGAGATGGACCGGGCCTTCGGCAACCG
CGCGCGTCACATCCGGCTTCGGCTGGGTGTTCTCAGGCAAGCGAAATGCCTGTCGGACGTCCCGACCGGCTCGCCGGACT
ACCGGCACATGCTGACGGGCGACCGGAAGGGCCAGTTCTCCGTCACGATCAGGGACAACTGGCGCATCGTCTTCGTGCCC
GACCACGACCCGGTCCCGTCCCTCCCGGACGGCGGCGTCGACGTCGCGAGCATCACGGCGGTCGAGATCGTCGAGGTCGT
GGACTACCACGGGAACTGA

Antitoxin


Download         Length: 365 a.a.        Molecular weight: 41193.89 Da        Isoelectric Point: 6.4487

>AT24169 WP_003602534.1 NC_012988:1124004-1125098 [Methylorubrum extorquens AM1]
MTRKEAFVPQWATHPGEHLAEYIEERGWSQAEFARLAGLTPKLVSTIINGTNPVTPETALRLEHVLGLKAYIWTGLQADW
DLFQARERERESAELQADWLKQFPVKELKACGRLPDTRDIGRLFGEMLTMLGIGTAQAYDARIGSLAVHHRRARSATASP
HHLYCWLMLGEERARQSELPAYDGYRFLQSCAEIRSLTVHGPDVFEPRMLELCRSAGVALVFQKPLGKTCVFGSARWMND
ERPLIQMSLRMKSNDHFWWTFFHEAAHIVLHRGKNFVDDQNGEGDGVEAEADAWAEEMLVGRERFEAFVTTCPSTKQEVA
AFAEEVGLHPGIVVGMLQHRRAVPFSHLNDLKARFAWADEVATA

Download         Length: 1095 bp

>AT24169 NC_012988:1124004-1125098 [Methylorubrum extorquens AM1]
ATGACGAGGAAGGAAGCCTTCGTCCCGCAATGGGCGACGCACCCCGGTGAGCACCTCGCGGAGTACATCGAGGAGCGCGG
CTGGTCGCAGGCCGAGTTCGCGCGCCTGGCCGGCCTGACGCCGAAGCTCGTCAGCACGATCATCAACGGCACGAACCCCG
TCACGCCGGAGACCGCCCTTCGGCTTGAGCATGTCCTCGGCCTGAAGGCCTACATCTGGACGGGGCTGCAGGCTGACTGG
GACCTGTTCCAGGCACGGGAGCGGGAGCGGGAGAGCGCCGAGCTCCAGGCCGACTGGCTGAAGCAGTTCCCCGTGAAGGA
ACTGAAGGCATGCGGCCGGCTCCCCGACACGCGCGACATCGGACGTCTGTTCGGCGAGATGCTGACAATGCTCGGCATCG
GCACCGCTCAGGCCTATGATGCCCGGATCGGATCGTTGGCGGTGCATCATCGCCGGGCGCGTTCCGCGACGGCGTCCCCG
CATCACCTGTACTGCTGGCTCATGCTGGGCGAGGAGCGAGCCCGCCAGAGCGAGCTTCCTGCCTACGACGGTTACCGGTT
CCTGCAGAGTTGCGCCGAGATCCGGTCCTTGACGGTGCACGGTCCGGATGTCTTCGAGCCGCGGATGCTGGAGCTTTGCC
GTTCGGCGGGCGTCGCCCTGGTATTCCAGAAGCCCCTGGGGAAGACCTGCGTCTTTGGATCGGCACGGTGGATGAACGAC
GAGCGGCCCCTGATCCAGATGTCGCTGCGCATGAAGAGCAACGACCATTTCTGGTGGACCTTCTTCCACGAGGCCGCGCA
CATCGTCCTGCATCGCGGGAAGAACTTCGTCGACGACCAGAACGGCGAGGGCGACGGCGTCGAGGCCGAGGCCGACGCCT
GGGCCGAGGAGATGCTGGTCGGGCGCGAGCGGTTCGAGGCCTTCGTGACGACGTGTCCCTCGACCAAGCAGGAGGTCGCC
GCCTTCGCCGAGGAGGTCGGCCTGCATCCGGGCATCGTGGTCGGCATGCTGCAGCATCGTCGCGCCGTGCCCTTCAGCCA
CCTCAACGACCTGAAGGCACGCTTCGCCTGGGCGGACGAGGTCGCGACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB C7CEU6

Antitoxin

Source ID Structure

References