Detailed information of TA system TA241676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3565598..3566255
• Replicon: chromosome
• Accession: NZ_CP095177
• Organism: Enterobacter hormaechei strain K528

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FDC1
• Locus tag: MUY31_RS17155
• Protein ID: WP_015571638.1
• Coordinates: 3565598..3565897 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MUY31_RS17160
• Protein ID: WP_048209500.1
• Coordinates: 3565908..3566255 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY31_RS17145 (MUY31_17145)	3561975..3564167	+	2193	WP_048210580.1	type I secretion system permease/ATPase	-
MUY31_RS17150 (MUY31_17150)	3564148..3565347	+	1200	WP_003862224.1	HlyD family efflux transporter periplasmic adaptor subunit	-
MUY31_RS17155 (MUY31_17155)	3565598..3565897	+	300	WP_015571638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MUY31_RS17160 (MUY31_17160)	3565908..3566255	+	348	WP_048209500.1	HigA family addiction module antitoxin	Antitoxin
MUY31_RS17165 (MUY31_17165)	3566331..3567512	-	1182	WP_023314694.1	PLP-dependent aminotransferase family protein	-
MUY31_RS17170 (MUY31_17170)	3567533..3568420	-	888	WP_048241982.1	LysR substrate-binding domain-containing protein	-
MUY31_RS17175 (MUY31_17175)	3568518..3569120	+	603	WP_023314696.1	short chain dehydrogenase	-
MUY31_RS17180 (MUY31_17180)	3569117..3569848	-	732	WP_023314697.1	class I SAM-dependent methyltransferase	-
MUY31_RS17185 (MUY31_17185)	3569923..3570921	-	999	WP_022651706.1	transketolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11624.29 Da
• Isoelectric Point: 9.6776

>T241676 WP_015571638.1 NZ_CP095177:3565598-3565897 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T241676 NZ_OD940420:c2867860-2867519 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACTCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAGATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13126.18 Da
• Isoelectric Point: 9.5932

>AT241676 WP_048209500.1 NZ_CP095177:3565908-3566255 [Enterobacter hormaechei]
MTLQQALRKPTMPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVA

Nucleotide

• Length: 348 bp

>AT241676 NZ_OD940420:c2868090-2867860 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A837FDC1