Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3099810..3100537 Replicon chromosome
Accession NC_012971
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ECD_RS15375 Protein ID WP_000550189.1
Coordinates 3100223..3100537 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECD_RS15370 Protein ID WP_000560266.1
Coordinates 3099810..3100226 (-) Length 139 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECD_RS15360 3094970..3097321 + 2352 WP_000695489.1 alpha-glucosidase -
ECD_RS15365 3097747..3099765 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ECD_RS15370 3099810..3100226 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ECD_RS15375 3100223..3100537 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ECD_RS15385 3100821..3101957 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ECD_RS15390 3102042..3102545 + 504 WP_001300832.1 M48 family metallopeptidase -
ECD_RS15395 3102622..3103314 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
ECD_RS15400 3103393..3104379 + 987 WP_012775974.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T24162 WP_000550189.1 NC_012971:c3100537-3100223 [Escherichia coli BL21(DE3)]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T24162 NC_012971:c3100537-3100223 [Escherichia coli BL21(DE3)]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT24162 WP_000560266.1 NC_012971:c3100226-3099810 [Escherichia coli BL21(DE3)]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT24162 NC_012971:c3100226-3099810 [Escherichia coli BL21(DE3)]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML


Antitoxin

Source ID Structure

References