Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 3035288..3035981 Replicon chromosome
Accession NC_012971
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag ECD_RS15060 Protein ID WP_000415584.1
Coordinates 3035685..3035981 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag ECD_RS15055 Protein ID WP_000650107.1
Coordinates 3035288..3035683 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECD_RS15045 3031152..3033410 - 2259 WP_001281877.1 DNA topoisomerase IV subunit A -
ECD_RS15050 3033548..3035155 - 1608 WP_001295629.1 ABC transporter substrate-binding protein -
ECD_RS15055 3035288..3035683 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
ECD_RS15060 3035685..3035981 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
ECD_RS15065 3036186..3036668 - 483 WP_000183505.1 transcriptional regulator -
ECD_RS15070 3036721..3037113 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
ECD_RS15075 3037265..3037924 + 660 WP_001221493.1 two-component system response regulator QseB -
ECD_RS15080 3037921..3039270 + 1350 WP_000673402.1 two-component system sensor histidine kinase QseC -
ECD_RS15085 3039316..3039648 - 333 WP_000917684.1 DUF2645 family protein -
ECD_RS15090 3039967..3040548 + 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
ECD_RS15095 3040579..3040893 + 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T24161 WP_000415584.1 NC_012971:c3035981-3035685 [Escherichia coli BL21(DE3)]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T24161 NC_012971:c3035981-3035685 [Escherichia coli BL21(DE3)]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT24161 WP_000650107.1 NC_012971:c3035683-3035288 [Escherichia coli BL21(DE3)]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT24161 NC_012971:c3035683-3035288 [Escherichia coli BL21(DE3)]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References