241581

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3860151..3860692 Replicon chromosome
Accession NZ_CP095155
Organism Escherichia coli strain Iso00041

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag MU410_RS18745 Protein ID WP_000615983.1
Coordinates 3860414..3860692 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag MU410_RS18740 Protein ID WP_000729703.1
Coordinates 3860151..3860411 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU410_RS18720 (3855826) 3855826..3856611 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MU410_RS18725 (3856583) 3856583..3858295 + 1713 Protein_3678 flagellar biosynthesis protein FlhA -
MU410_RS18730 (3858519) 3858519..3859016 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
MU410_RS18735 (3859192) 3859192..3859941 - 750 WP_000056849.1 C40 family peptidase -
MU410_RS18740 (3860151) 3860151..3860411 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MU410_RS18745 (3860414) 3860414..3860692 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MU410_RS18750 (3860848) 3860848..3861588 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MU410_RS18755 (3861559) 3861559..3862326 - 768 WP_000333380.1 class II glutamine amidotransferase -
MU410_RS18760 (3862532) 3862532..3863110 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3835692..3869234 33542


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T241581 WP_000615983.1 NZ_CP095155:3860414-3860692 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T241581 NZ_LT992489:452348-452617 [Vibrio cholerae]
ATGTATAAACTCGAATACTCCACACAGTTTAAGAAAGATTTCAAAAAGATAACTAAGATGCCGATTTCAGACATCATTGA
AGTCGGCAATGTTATTTCTAAGCTGCAACGCGGCGAAAAGCTTGAACCCAAGAATGTTGACCATCCGTTAACTGGAAATT
GGGTTGGATTTCGAGACTGCCACATTAAACCCGACCTAGTGTTAATCTATCGAGTTTTTAACGATCAACTACAACTAGCG
CGCATTGGTTCACATAGTGATTTATTCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT241581 WP_000729703.1 NZ_CP095155:3860151-3860411 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT241581 NZ_LT992489:452077-452355 [Vibrio cholerae]
ATGAGAACAGAAATGCTGAGCACGCGCATTGACCACGACACGAAAATTGCGTTCACAAACGTCTGCGATGAGATGGGTCT
AAGTACATCACAGGCCATAAAGTTATTTGCAAAAGCGGTTATTAATCATGGTGGAATCCCTTTTGAGCTGCGAGTTCCAC
AGCCTAATGAGGTTACTGCATCTGCAATTCAAGAGCTCGTTGAAGGAAAAGGACATAAAGCTGAATCCGTTGAAGCTATG
CTGAATGAGCTTACTGAAGGCAAAGTTAAGCATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1F716

References