Detailed information of TA system TA241566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1106204..1106931
• Replicon: chromosome
• Accession: NZ_CP095155
• Organism: Escherichia coli strain Iso00041

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: MU410_RS05405
• Protein ID: WP_001521139.1
• Coordinates: 1106204..1106515 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MU410_RS05410
• Protein ID: WP_000126294.1
• Coordinates: 1106512..1106931 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU410_RS05380 (1102139)	1102139..1103848	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
MU410_RS05385 (1103858)	1103858..1104400	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
MU410_RS05390 (1104400)	1104400..1105167	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
MU410_RS05395 (1105164)	1105164..1105574	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
MU410_RS05400 (1105567)	1105567..1106037	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
MU410_RS05405 (1106204)	1106204..1106515	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
MU410_RS05410 (1106512)	1106512..1106931	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
MU410_RS05415 (1107010)	1107010..1108434	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
MU410_RS05420 (1108443)	1108443..1109900	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
MU410_RS05425 (1110160)	1110160..1111170	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
MU410_RS05430 (1111319)	1111319..1111846	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T241566 WP_001521139.1 NZ_CP095155:1106204-1106515 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T241566 NZ_LT992488:2401853-2402176 [Vibrio cholerae]
ATGAGCTGGAAGATCGACTTTTACGATGGCGTTGAGGATCAGATCCTCGATATGCCACCCAAAATACAAGCTCGCATGAT
TAAGCTTTTAGAGCTGATGGAAAAGCATGGTGCAAACTTGGGGCCTCCTCATACTGAGTCTATGGGCGATGGGTTATTTG
AAATCCGCGCCAAAGCTCAAGAAGGTATTGGTAGAGGCTTATTTTGCTACTTAAAAGGGAATCACATTTATGTGTTACAC
GCTTTTGTAAAAAAATCTCAAAAGACGCCCAAAAATGAGCTCAATCTTGCCAGAGATAGACAAAAAGAGGTTCAAAAATC
ATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT241566 WP_000126294.1 NZ_CP095155:1106512-1106931 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT241566 NZ_LT992488:2402173-2402451 [Vibrio cholerae]
ATGAGTCTGCAAAAACTGAAACAACGTGCTTTAGCCAATGCTGAAGTTAAGAATGAGTACGATAAGCTTGAGAGTGAGTT
CAGCTTTATTGATCAATTGCTTACCATGAGAACAAAGGCAGGACTTACTCAAGAACAAGTTGCTGAAAGAATGCATACCC
AAAAAAGTAATGTCAGTCGTTTAGAAAAAGGTAATGCCAATCCAAGTTGGTCAACTCTTCTAAAATATGCTCACGCATGT
GGCTTTGAACTAACACTCAAGCCACAAAAAATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A829L2T9