Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 646300..647099 Replicon chromosome
Accession NZ_CP095155
Organism Escherichia coli strain Iso00041

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag MU410_RS03165 Protein ID WP_000347272.1
Coordinates 646300..646764 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MU410_RS03170 Protein ID WP_001307405.1
Coordinates 646764..647099 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU410_RS03135 (641301) 641301..641735 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MU410_RS03140 (641753) 641753..642631 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
MU410_RS03145 (642621) 642621..643400 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MU410_RS03150 (643411) 643411..643884 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MU410_RS03155 (643907) 643907..645187 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
MU410_RS03160 (645436) 645436..646245 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
MU410_RS03165 (646300) 646300..646764 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MU410_RS03170 (646764) 646764..647099 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MU410_RS03175 (647248) 647248..648819 - 1572 WP_001273738.1 galactarate dehydratase -
MU410_RS03180 (649194) 649194..650528 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
MU410_RS03185 (650544) 650544..651314 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T241562 WP_000347272.1 NZ_CP095155:c646764-646300 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T241562 NZ_LT992487:970548-970817 [Vibrio cholerae]
ATGTATAAACTCGAATACTCCACACAGTTTAAGAAAGATTTCAAAAAGATAACTAAGATGCCGATTTCAGACATCATTGA
AGTCGGCAATGTTATTTCTAAGCTGCAACGCGGCGAAAAGCTTGAACCCAAGAATGTTGACCATCCGTTAACTGGAAATT
GGGTTGGATTTCGAGACTGCCACATTAAACCCGACCTAGTGTTAATCTATCGAGTTTTTAACGATCAACTACAACTAGCG
CGCATTGGTTCACATAGTGATTTATTCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT241562 WP_001307405.1 NZ_CP095155:c647099-646764 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT241562 NZ_LT992487:970277-970555 [Vibrio cholerae]
ATGAGAACAGAAATGCTGAGCACGCGCATTGACCACGACACGAAAATTGCGTTCACAAACGTCTGCGATGAGATGGGTCT
AAGTACATCACAGGCCATAAAGTTATTTGCAAAAGCGGTTATTAATCATGGTGGAATCCCTTTTGAGCTGCGAGTTCCAC
AGCCTAATGAGGTTACTGCATCTGCAATTCAAGAGCTCGTTGAAGGAAAAGGACATAAAGCTGAATCCGTTGAAGCTATG
CTGAATGAGCTTACTGAAGGCAAAGTTAAGCATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References