Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 331627..332213 Replicon chromosome
Accession NZ_CP095150
Organism Klebsiella pneumoniae strain Iso00073

Toxin (Protein)


Gene name doc Uniprot ID A0A483YV82
Locus tag MU411_RS01545 Protein ID WP_019725145.1
Coordinates 331845..332213 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A483YZZ9
Locus tag MU411_RS01540 Protein ID WP_019725146.1
Coordinates 331627..331848 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU411_RS01520 327784..328710 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MU411_RS01525 328707..329984 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MU411_RS01530 329981..330748 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MU411_RS01535 330750..331463 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MU411_RS01540 331627..331848 + 222 WP_019725146.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MU411_RS01545 331845..332213 + 369 WP_019725145.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MU411_RS01550 332485..333801 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MU411_RS01555 333908..334795 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MU411_RS01560 334792..335637 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MU411_RS01565 335639..336709 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 328707..337446 8739


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13542.89 Da        Isoelectric Point: 8.6410

>T241540 WP_019725145.1 NZ_CP095150:331845-332213 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTSGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFIKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T241540 NZ_LT992477:2015217-2015324 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT241540 WP_019725146.1 NZ_CP095150:331627-331848 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDELRELADK

Download         Length: 222 bp

>AT241540 NZ_LT992477:c2015401-2015341 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483YV82

Antitoxin

Source ID Structure
AlphaFold DB A0A483YZZ9

References