Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3593328..3594007 Replicon chromosome
Accession NZ_CP095137
Organism Escherichia coli strain Iso00225

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag MU414_RS17660 Protein ID WP_000057523.1
Coordinates 3593705..3594007 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag MU414_RS17655 Protein ID WP_000806442.1
Coordinates 3593328..3593669 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU414_RS17645 (3589572) 3589572..3590504 - 933 WP_000883041.1 glutaminase A -
MU414_RS17650 (3590766) 3590766..3593270 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
MU414_RS17655 (3593328) 3593328..3593669 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
MU414_RS17660 (3593705) 3593705..3594007 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MU414_RS17665 (3594140) 3594140..3594934 + 795 WP_000365147.1 TraB/GumN family protein -
MU414_RS17670 (3595138) 3595138..3595617 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
MU414_RS17675 (3595641) 3595641..3596441 + 801 WP_000439798.1 hypothetical protein -
MU414_RS17680 (3596438) 3596438..3596941 + 504 WP_000667000.1 hypothetical protein -
MU414_RS17685 (3596979) 3596979..3598631 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T241489 WP_000057523.1 NZ_CP095137:c3594007-3593705 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T241489 NZ_LT992471:c2626859-2626752 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT241489 WP_000806442.1 NZ_CP095137:c3593669-3593328 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT241489 NZ_LT992471:2626675-2626735 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References