Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1971130..1971632 Replicon chromosome
Accession NZ_CP095137
Organism Escherichia coli strain Iso00225

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag MU414_RS09365 Protein ID WP_000767822.1
Coordinates 1971378..1971632 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MU414_RS09360 Protein ID WP_001259255.1
Coordinates 1971130..1971381 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU414_RS09335 (1966308) 1966308..1967375 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
MU414_RS09340 (1967375) 1967375..1968445 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
MU414_RS09345 (1968442) 1968442..1969746 - 1305 WP_001546022.1 histidinol dehydrogenase -
MU414_RS09350 (1969752) 1969752..1970651 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
MU414_RS09355 (1970797) 1970797..1970847 - 51 WP_001364200.1 his operon leader peptide -
MU414_RS09360 (1971130) 1971130..1971381 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MU414_RS09365 (1971378) 1971378..1971632 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MU414_RS09370 (1971715) 1971715..1972539 + 825 WP_000754737.1 SDR family oxidoreductase -
MU414_RS09375 (1972585) 1972585..1973514 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
MU414_RS09380 (1973729) 1973729..1973791 + 63 WP_010723108.1 membrane protein YoeI -
MU414_RS09385 (1973781) 1973781..1975139 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T241478 WP_000767822.1 NZ_CP095137:1971378-1971632 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T241478 NZ_LT992470:2599017-2599193 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT241478 WP_001259255.1 NZ_CP095137:1971130-1971381 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT241478 NZ_LT992470:c2599355-2599181 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References