Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 649251..650050 Replicon chromosome
Accession NZ_CP095137
Organism Escherichia coli strain Iso00225

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag MU414_RS03215 Protein ID WP_000347251.1
Coordinates 649251..649715 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag MU414_RS03220 Protein ID WP_001296435.1
Coordinates 649715..650050 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU414_RS03185 (644252) 644252..644686 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MU414_RS03190 (644704) 644704..645582 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MU414_RS03195 (645572) 645572..646351 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MU414_RS03200 (646362) 646362..646835 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MU414_RS03205 (646858) 646858..648138 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
MU414_RS03210 (648387) 648387..649196 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MU414_RS03215 (649251) 649251..649715 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MU414_RS03220 (649715) 649715..650050 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MU414_RS03225 (650199) 650199..651770 - 1572 WP_001273763.1 galactarate dehydratase -
MU414_RS03230 (652145) 652145..653479 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
MU414_RS03235 (653495) 653495..654265 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T241474 WP_000347251.1 NZ_CP095137:c649715-649251 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T241474 NZ_LT992470:2130658-2130765 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT241474 WP_001296435.1 NZ_CP095137:c650050-649715 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT241474 NZ_LT992470:c2130842-2130782 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References