Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 332037..332623 Replicon chromosome
Accession NZ_CP095132
Organism Klebsiella pneumoniae strain Iso00267

Toxin (Protein)


Gene name doc Uniprot ID A0A486RB10
Locus tag MU415_RS01555 Protein ID WP_004174007.1
Coordinates 332255..332623 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MU415_RS01550 Protein ID WP_004174006.1
Coordinates 332037..332258 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU415_RS01530 (328194) 328194..329120 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MU415_RS01535 (329117) 329117..330394 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MU415_RS01540 (330391) 330391..331158 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MU415_RS01545 (331160) 331160..331873 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MU415_RS01550 (332037) 332037..332258 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MU415_RS01555 (332255) 332255..332623 + 369 WP_004174007.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MU415_RS01560 (332896) 332896..334212 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MU415_RS01565 (334319) 334319..335206 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MU415_RS01570 (335203) 335203..336048 + 846 WP_004174009.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MU415_RS01575 (336050) 336050..337120 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 329117..337857 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13568.97 Da        Isoelectric Point: 8.6410

>T241449 WP_004174007.1 NZ_CP095132:332255-332623 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDLGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T241449 NZ_LT992467:c1066961-1066854 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT241449 WP_004174006.1 NZ_CP095132:332037-332258 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT241449 NZ_LT992467:1066777-1066837 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486RB10

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References