Detailed information of TA system TA241446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 56467..57179
• Replicon: plasmid pIso00270_02
• Accession: NZ_CP095127
• Organism: Escherichia coli strain Iso00270

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: MU416_RS23385
• Protein ID: WP_000162415.1
• Coordinates: 56877..57179 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MU416_RS23380
• Protein ID: WP_032220986.1
• Coordinates: 56467..56805 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU416_RS23345	51624..52016	-	393	WP_000824759.1	hypothetical protein	-
MU416_RS23350	52037..52660	-	624	WP_202117614.1	hypothetical protein	-
MU416_RS23355	52929..53582	+	654	WP_001557715.1	hypothetical protein	-
MU416_RS23360	53911..54240	+	330	WP_000542383.1	hypothetical protein	-
MU416_RS23365	54233..55426	+	1194	WP_097433648.1	phage tail protein	-
MU416_RS23370	55460..56188	-	729	WP_197347751.1	hypothetical protein	-
MU416_RS23375	56225..56410	-	186	Protein_73	hypothetical protein	-
MU416_RS23380	56467..56805	-	339	WP_032220986.1	HigA family addiction module antitoxin	Antitoxin
MU416_RS23385	56877..57179	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MU416_RS23390	57342..58133	+	792	WP_197347752.1	phage tail protein	-
MU416_RS23395	58130..58897	+	768	WP_197347753.1	baseplate	-
MU416_RS23400	58901..59881	+	981	WP_000046500.1	hypothetical protein	-
MU416_RS23405	59878..60531	+	654	WP_197347754.1	baseplate protein	-
MU416_RS23410	60591..61496	+	906	WP_080028513.1	recombination-associated protein RdgC	-
MU416_RS23415	61540..62160	+	621	WP_044862276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94223	94223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T241446 WP_000162415.1 NZ_CP095127:c57179-56877 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T241446 NZ_LT992467:c687342-686980 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13068.01 Da
• Isoelectric Point: 6.4754

>AT241446 WP_032220986.1 NZ_CP095127:c56805-56467 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNMNKKAA

Nucleotide

• Length: 339 bp

>AT241446 NZ_LT992467:c687509-687339 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3