Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3650591..3651285 Replicon chromosome
Accession NZ_CP095125
Organism Escherichia coli strain Iso00270

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag MU416_RS17635 Protein ID WP_001263489.1
Coordinates 3650591..3650989 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag MU416_RS17640 Protein ID WP_000554758.1
Coordinates 3650992..3651285 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3646179) 3646179..3646259 - 81 NuclAT_12 - -
- (3646179) 3646179..3646259 - 81 NuclAT_12 - -
- (3646179) 3646179..3646259 - 81 NuclAT_12 - -
- (3646179) 3646179..3646259 - 81 NuclAT_12 - -
MU416_RS17610 (3646855) 3646855..3647313 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
MU416_RS17615 (3647574) 3647574..3649031 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
MU416_RS17620 (3649088) 3649088..3649609 - 522 Protein_3459 peptide chain release factor H -
MU416_RS17625 (3649605) 3649605..3649811 - 207 Protein_3460 RtcB family protein -
MU416_RS17630 (3650129) 3650129..3650581 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
MU416_RS17635 (3650591) 3650591..3650989 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MU416_RS17640 (3650992) 3650992..3651285 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MU416_RS17645 (3651337) 3651337..3652392 - 1056 WP_001226164.1 DNA polymerase IV -
MU416_RS17650 (3652463) 3652463..3653248 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MU416_RS17655 (3653220) 3653220..3654932 + 1713 Protein_3466 flagellar biosynthesis protein FlhA -
MU416_RS17660 (3655156) 3655156..3655653 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T241440 WP_001263489.1 NZ_CP095125:c3650989-3650591 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T241440 NZ_LT992466:1054663-1054839 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT241440 WP_000554758.1 NZ_CP095125:c3651285-3650992 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT241440 NZ_LT992466:c1055001-1054827 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References