Detailed information of TA system TA024138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2500785..2501383
• Replicon: chromosome
• Accession: NC_012969
• Organism: Methylovorus glucosetrophus SIP3-4

Toxin (Protein)

• Gene name: graT
• Uniprot ID: C6XA68
• Locus tag: MSIP34_RS11900
• Protein ID: WP_015830899.1
• Coordinates: 2501102..2501383 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: C6XA67
• Locus tag: MSIP34_RS11895
• Protein ID: WP_015830898.1
• Coordinates: 2500785..2501090 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSIP34_RS11875	2496966..2497724	+	759	WP_015830894.1	membrane protein	-
MSIP34_RS11880	2497743..2498495	+	753	WP_148211187.1	hypothetical protein	-
MSIP34_RS11885	2498509..2499174	+	666	WP_015830896.1	response regulator transcription factor	-
MSIP34_RS11890	2499150..2500496	+	1347	WP_015830897.1	HAMP domain-containing histidine kinase	-
MSIP34_RS11895	2500785..2501090	-	306	WP_015830898.1	HigA family addiction module antidote protein	Antitoxin
MSIP34_RS11900	2501102..2501383	-	282	WP_015830899.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MSIP34_RS14975	2501642..2501833	+	192	WP_083773324.1	type II toxin-antitoxin system MqsA family antitoxin	-
MSIP34_RS11905	2501883..2502104	-	222	WP_015830900.1	hypothetical protein	-
MSIP34_RS11910	2502108..2502461	-	354	WP_041370182.1	hypothetical protein	-
MSIP34_RS11915	2502577..2502756	-	180	WP_041370184.1	hypothetical protein	-
MSIP34_RS11920	2502763..2503041	-	279	WP_015830902.1	addiction module antidote protein	-
MSIP34_RS11925	2503105..2503497	-	393	WP_015830903.1	hypothetical protein	-
MSIP34_RS11930	2503508..2504626	-	1119	WP_015830904.1	type IV secretion system protein	-
MSIP34_RS11935	2504653..2504973	-	321	WP_015830905.1	hypothetical protein	-
MSIP34_RS15280	2504970..2505227	-	258	WP_041370186.1	hypothetical protein	-
MSIP34_RS11945	2505224..2506261	-	1038	WP_015830906.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (24-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-96)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10835.32 Da
• Isoelectric Point: 6.7097

>T24138 WP_015830899.1 NC_012969:c2501383-2501102 [Methylovorus glucosetrophus SIP3-4]
MTIQTFLCQDTQDIYEGKHPRRFRNIEAVAERKLQMLDAAVELKDLRSPPGNRLEALIGDRAGQHSIRINDQWRICFVWT
ATGPDRVEIVDYH

Nucleotide

• Length: 282 bp

>T24138 NC_012969:c2501383-2501102 [Methylovorus glucosetrophus SIP3-4]
ATGACGATCCAAACATTCCTGTGCCAGGACACCCAAGATATTTATGAAGGGAAACATCCTAGAAGGTTCAGAAATATCGA
GGCAGTCGCCGAGCGAAAGCTTCAAATGCTGGATGCAGCTGTCGAGCTGAAAGATTTGCGTTCACCGCCAGGCAATAGGC
TGGAAGCGCTAATCGGTGATCGGGCTGGTCAGCACAGCATACGCATCAATGACCAGTGGCGCATTTGCTTTGTGTGGACA
GCGACAGGCCCTGATCGTGTTGAGATCGTCGATTACCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11477.18 Da
• Isoelectric Point: 7.3190

>AT24138 WP_015830898.1 NC_012969:c2501090-2500785 [Methylovorus glucosetrophus SIP3-4]
MATNNMRPIHPGEILREEYLVPLEMSGHALSIELRVPATKINDILRARRAVTSDTALRLSRFFGTTPQFWANLQSAYDLK
QAEIAHGKEIEQQVRQMDMAA

Nucleotide

• Length: 306 bp

>AT24138 NC_012969:c2501090-2500785 [Methylovorus glucosetrophus SIP3-4]
ATGGCAACCAACAATATGAGACCAATTCATCCGGGCGAGATTCTGCGCGAGGAGTATCTTGTGCCTCTCGAAATGAGCGG
TCATGCCCTTTCTATTGAGCTGCGCGTCCCCGCGACTAAAATCAATGACATTTTGCGAGCACGCCGTGCTGTTACATCTG
ACACCGCTTTGCGGTTGTCGCGGTTCTTCGGAACGACCCCGCAGTTTTGGGCAAACCTGCAAAGTGCATATGATTTGAAG
CAGGCTGAGATAGCCCATGGCAAAGAGATCGAGCAGCAGGTCAGACAGATGGATATGGCCGCTTAG

Structures

Toxin

• AlphaFold DB: C6XA68

Antitoxin

• AlphaFold DB: C6XA67