Detailed information of TA system TA241320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 10396..10919
• Replicon: plasmid unnamed
• Accession: NZ_CP095103
• Organism: Staphylococcus simulans strain IVB6188

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: D3X7L5
• Locus tag: MUA56_RS12530
• Protein ID: WP_013012280.1
• Coordinates: 10396..10662 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: D3X7L6
• Locus tag: MUA56_RS12535
• Protein ID: WP_013012281.1
• Coordinates: 10662..10919 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA56_RS12500 (MUA56_12500)	5700..6374	-	675	WP_262551432.1	IS6 family transposase	-
MUA56_RS12505 (MUA56_12505)	6677..6925	-	249	WP_262551433.1	hypothetical protein	-
MUA56_RS12510 (MUA56_12510)	6939..7601	-	663	WP_262551434.1	hypothetical protein	-
MUA56_RS12515 (MUA56_12515)	7620..8240	-	621	WP_262551435.1	hypothetical protein	-
MUA56_RS12520 (MUA56_12520)	9125..9598	-	474	Protein_12	sodium:glutamate symporter	-
MUA56_RS12525 (MUA56_12525)	9652..10327	+	676	Protein_13	IS6 family transposase	-
MUA56_RS12530 (MUA56_12530)	10396..10662	-	267	WP_013012280.1	Txe/YoeB family addiction module toxin	Toxin
MUA56_RS12535 (MUA56_12535)	10662..10919	-	258	WP_013012281.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MUA56_RS12540 (MUA56_12540)	11259..11354	+	96	WP_262551436.1	type I toxin-antitoxin system Fst family toxin	-
MUA56_RS12545 (MUA56_12545)	11842..12234	+	393	WP_262551437.1	IS6 family transposase	-
MUA56_RS12550 (MUA56_12550)	12441..12617	+	177	WP_262551438.1	hypothetical protein	-
MUA56_RS12555 (MUA56_12555)	12645..13013	+	369	WP_262551439.1	hypothetical protein	-
MUA56_RS12560 (MUA56_12560)	13700..15016	+	1317	WP_061054687.1	ISL3 family transposase	-
MUA56_RS12565 (MUA56_12565)	15351..15716	+	366	WP_262551440.1	replication/maintenance protein RepL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55879	55879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10601.09 Da
• Isoelectric Point: 9.9090

>T241320 WP_013012280.1 NZ_CP095103:c10662-10396 [Staphylococcus simulans]
MSYYSVLIKNSAKSDLKKINKSHLKEQFLNVVETLKINPYEPSQSFEKLQPKHLGRYSRRINHQHRIVYTVDDEKKEVYI
FSAWSHYE

Nucleotide

• Length: 267 bp

>T241320 NZ_LT991956:179188-180513 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAGCCGGAAACAGCAGCGCCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGACGAACCCACAGATTTATATTCCGTGAAGTGTCACCCTCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGCATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTACTGAGTAATTCAGACCGCGCAGAGC
ATGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTGTTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCAAGG
GAACAATCAGATATCCTTGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACGGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGAGTCATTGAACGTGTTAGCGGGCTTCTTCCGTACCAGTTCCACCAGCAGCTTGTCGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9813.90 Da
• Isoelectric Point: 4.6263

>AT241320 WP_013012281.1 NZ_CP095103:c10919-10662 [Staphylococcus simulans]
MNIKTPTNARKEFYELLKHVNVNHEPVIISGSKSENNAVIVSQKDWDSIQETLYLESVGVMDKVREREKDSSSYTNIDDL
DWSNL

Nucleotide

• Length: 258 bp

>AT241320 NZ_LT991956:178890-179183 [Enterobacter hormaechei subsp. steigerwaltii]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Structures

Toxin

• AlphaFold DB: D3X7L5

Antitoxin

• AlphaFold DB: D3X7L6