Detailed information of TA system TA241304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 6200..6851
• Replicon: plasmid pAB4451
• Accession: NZ_CP095092
• Organism: Acinetobacter baumannii strain AB4451

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: MUU69_RS18965
• Protein ID: WP_000269904.1
• Coordinates: 6495..6851 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MUU69_RS18960
• Protein ID: WP_001129974.1
• Coordinates: 6200..6502 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUU69_RS18930 (MUU69_18930)	1217..1360	+	144	WP_245113211.1	hypothetical protein	-
MUU69_RS18935 (MUU69_18935)	1784..2071	+	288	WP_000438827.1	BrnT family toxin	-
MUU69_RS18940 (MUU69_18940)	2058..2372	+	315	WP_001983304.1	BrnA antitoxin family protein	-
MUU69_RS18945 (MUU69_18945)	2500..4906	+	2407	Protein_6	TonB-dependent receptor ZnuD2	-
MUU69_RS18950 (MUU69_18950)	5211..5672	+	462	WP_000761346.1	DIP1984 family protein	-
MUU69_RS18955 (MUU69_18955)	5998..6207	-	210	WP_000069474.1	hypothetical protein	-
MUU69_RS18960 (MUU69_18960)	6200..6502	-	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
MUU69_RS18965 (MUU69_18965)	6495..6851	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MUU69_RS18970 (MUU69_18970)	7417..7755	-	339	WP_245113200.1	hypothetical protein	-
MUU69_RS18975 (MUU69_18975)	7831..8592	-	762	WP_000425105.1	hypothetical protein	-
MUU69_RS18980 (MUU69_18980)	8895..9719	+	825	WP_245113202.1	MobA/MobL family protein	-
MUU69_RS18985 (MUU69_18985)	9889..10062	+	174	WP_228200477.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11178	11178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T241304 WP_000269904.1 NZ_CP095092:c6851-6495 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T241304 NZ_LT991954:c103682-103362 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCACATTTCAACTGTACCGGCCAAGGTGCCGGTTTCGTCACGTCTGTCGCCCGTGCAGGTCTGGCAGCAACTTTTAAC
GTATCTGCTGGGTCACCATTACGGCCTGACGCTTAACGACACACCATTCCATGACAACGCGGCCATCGAAGAGCATATCG
ATGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGCATCGACCGCAAAGGGTTT
AGCTGGCAGGAACAGACTCCGTTCCTGACCGCGACTGATATTCTCCGAGCCAGGCGAGCTACCGGATTAATAAATACTTA
A

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT241304 WP_001129974.1 NZ_CP095092:c6502-6200 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT241304 NZ_LT991954:c104042-103719 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTCAATCACACCACCTCATGAATGGGGTCTACAAAGCGATATCACCCCTCGATTTGGCGCAAGATTAGTTCAGGAAGG
CAACCGGCTGTATTACCTGGCTGACAGGGCTGGATTGACGGGGAGTTTTAGCCCAATGCACCTGCAGAAATTAGATCTGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAGATATGCTGCGTGCTGGCGAACTAAATCCACATCAGCAATGTCAGGTGAAA
ATTCAACTTGCGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGTGGTTACGTTTACATCTCTATTTATCCCAAACC
GTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1