241296

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4235938..4236435 Replicon chromosome
Accession NZ_CP095088
Organism Escherichia coli isolate w264

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MUK78_RS20795 Protein ID WP_001173343.1
Coordinates 4235938..4236111 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MUK78_RS20800 Protein ID WP_000398619.1
Coordinates 4236139..4236435 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK78_RS20780 (4231289) 4231289..4232746 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
MUK78_RS20785 (4232983) 4232983..4234500 + 1518 WP_001295074.1 lysine--tRNA ligase -
MUK78_RS20790 (4234672) 4234672..4235900 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
MUK78_RS20795 (4235938) 4235938..4236111 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MUK78_RS20800 (4236139) 4236139..4236435 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MUK78_RS20805 (4236662) 4236662..4236934 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MUK78_RS20810 (4236946) 4236946..4237176 - 231 WP_024250366.1 4Fe-4S mono-cluster protein YjdI -
MUK78_RS20815 (4237357) 4237357..4238988 + 1632 WP_001216477.1 sensor histidine kinase -
MUK78_RS20820 (4238985) 4238985..4239704 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4227149..4236435 9286


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T241296 WP_001173343.1 NZ_CP095088:c4236111-4235938 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T241296 NZ_LT985192:135393-135743 [Pseudomonas syringae]
ATGAATACGTTTGCACTGCGCTTTACCGATCTTGCCCAGCAAAGCCTTGAAGACCAGGTTGAACACTTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATTCCCTTATCGATGCCATCCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCACAGTTGAGTGAGCTGGGTGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGGATCTTTTAT
GAGGTGATGGATTCTGATGGTATTACCGATATCGCCGTATTGCTCGTATTAGGCGGGAAACAAAGTGTTGAACAAGCCCT
CATCCGCTATTGCCTTCTTCAGCCTATATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT241296 WP_000398619.1 NZ_CP095088:c4236435-4236139 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT241296 NZ_LT985192:135132-135380 [Pseudomonas syringae]
ATGCGAGTTGAAACAATAAGCTACCTGAAACGCAATGCAGCCGACCTGCCTCTCGATGAGCCATTGATCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCGGATCGTAAGCGCCGCGATGAGGCCATTGCCTTGGTAAAGCTCTTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTGCC
CACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References