Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3739359..3739900 Replicon chromosome
Accession NZ_CP095088
Organism Escherichia coli isolate w264

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag MUK78_RS18380 Protein ID WP_000615979.1
Coordinates 3739622..3739900 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag MUK78_RS18375 Protein ID WP_000729704.1
Coordinates 3739359..3739619 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK78_RS18355 (3735042) 3735042..3735827 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
MUK78_RS18360 (3735799) 3735799..3737511 + 1713 Protein_3606 flagellar biosynthesis protein FlhA -
MUK78_RS18365 (3737727) 3737727..3738224 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
MUK78_RS18370 (3738400) 3738400..3739173 - 774 WP_001353765.1 C40 family peptidase -
MUK78_RS18375 (3739359) 3739359..3739619 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MUK78_RS18380 (3739622) 3739622..3739900 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MUK78_RS18385 (3740056) 3740056..3740796 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MUK78_RS18390 (3740767) 3740767..3741534 - 768 WP_000333379.1 class II glutamine amidotransferase -
MUK78_RS18395 (3741740) 3741740..3742318 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T241293 WP_000615979.1 NZ_CP095088:3739622-3739900 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T241293 NZ_LT985188:1776082-1776432 [Micropruina glycogenica]
CTGATGCTGCGAGGTGAGATCCGCCTGACTGACCTGGACCCCGCCAGGGCCGCGGAGGCCGACACGCGACGGCCCGCGAT
CATCGTCAGCAACGACCGCGCCAACGCGACCGCTGCCAGGTTGGGCCGAGGGGTGGTCACGGTTGTGCCGATCACTAGCA
ACATCTCCCGCGTCTTCCCGTTCCAGGTATTGCTGCCCGCTGATGACGTCGGGATCCGGGTGGACTCCAAGGCTCAGGCC
GAACAGGTCCGCTCGGTGTCGATCGAACGCATCGGCCCGGTGATCGGCCGCCTGCCCACGCACCTGATCAGCAAGCTCGA
CGACGCCCTGCGGCTCCATCTCCAGCTCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT241293 WP_000729704.1 NZ_CP095088:3739359-3739619 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT241293 NZ_LT985188:1775867-1776085 [Micropruina glycogenica]
GTGAAGTTGAGTGTGAGCCTCTCGCCGAGCGAGGTCGAAACGCTGGACAAGTACGCCAGGGCGGCAGGTCTGAAGTCTCG
GTCGGCGGCAATCCAGCAGGCGATCAAGTTGCTCGGAGATCCCGAGTTGGAGGACGCCTATGCCGCGGCGTGGCAGGAGT
GGGAGGACTCCGGGGAATCTGAGGCGTGGGCGGGCACCGTCGCTGACGGGCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References