Detailed information of TA system TA241292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3733170..3733864
• Replicon: chromosome
• Accession: NZ_CP095088
• Organism: Escherichia coli isolate w264

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: MUK78_RS18340
• Protein ID: WP_089578406.1
• Coordinates: 3733170..3733568 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: MUK78_RS18345
• Protein ID: WP_000554757.1
• Coordinates: 3733571..3733864 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3728830)	3728830..3728910	-	81	NuclAT_10	-	-
- (3728830)	3728830..3728910	-	81	NuclAT_10	-	-
- (3728830)	3728830..3728910	-	81	NuclAT_10	-	-
- (3728830)	3728830..3728910	-	81	NuclAT_10	-	-
MUK78_RS18310 (3728170)	3728170..3729414	-	1245	WP_000189541.1	esterase FrsA	-
MUK78_RS18315 (3729506)	3729506..3729964	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
MUK78_RS18320 (3730225)	3730225..3731682	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
MUK78_RS18325 (3731739)	3731739..3732260	-	522	Protein_3599	peptide chain release factor H	-
MUK78_RS18330 (3732259)	3732259..3732462	-	204	Protein_3600	RtcB family protein	-
MUK78_RS18335 (3732708)	3732708..3733160	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
MUK78_RS18340 (3733170)	3733170..3733568	-	399	WP_089578406.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MUK78_RS18345 (3733571)	3733571..3733864	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MUK78_RS18350 (3733916)	3733916..3734971	-	1056	WP_001226164.1	DNA polymerase IV	-
MUK78_RS18355 (3735042)	3735042..3735827	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
MUK78_RS18360 (3735799)	3735799..3737511	+	1713	Protein_3606	flagellar biosynthesis protein FlhA	-
MUK78_RS18365 (3737727)	3737727..3738224	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15506.85 Da
• Isoelectric Point: 8.0949

>T241292 WP_089578406.1 NZ_CP095088:c3733568-3733170 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHFVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T241292 NZ_LT985188:971559-971831 [Micropruina glycogenica]
GTGCCGGGCCCCTATGAGGTCGTGTTCACGAGGACGGCCCGTCGGGCACTCGAGCGGGATCTCCCCGAGAAGGTCGCGGC
GGCCGCCTTCGAGTTCATCATGGGCGCTCTGCGCGCGAACCCACGGCGAGTCGGCAAGCCTCTGCGTGAACCGCTAGCTC
CGCTGTATGCGGCCCGGCGGGGCGAGTACCGGGTGATCTACCGAATCGTCGATCAGCGTCTCGTCATCGAGATCGTCTCG
GTCGTGCATCGCCGGGACGCGTACCACTCGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT241292 WP_000554757.1 NZ_CP095088:c3733864-3733571 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT241292 NZ_LT985188:971278-971553 [Micropruina glycogenica]
ATGACCACACTGTCACTGGCTGAGGCCCGGGCCAACCTATCCAAGCTTGTGGAGTCGGCCGTGTCAACGCACGAGCGGTT
CGAGGTGACGCGCAACGGCGACCGGGTAGCAGTCCTGCTCGGTGCGGATGACTACGACTCCCTGCTGGAGACGCTCGACA
TCCTGAGCAGTCCCGATGAGGTCGAGGCGATCCGCGCAGGTCTGGACGATCTGGAGAGCGGAGAGGTCAGTTCCGCGGAC
GAGGTTCGTGCCGCGCTGGCTGCACGCGGAAAGTAG

Structures

Antitoxin

• AlphaFold DB: S1FJN6