Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 672537..673264 Replicon chromosome
Accession NZ_CP095088
Organism Escherichia coli isolate w264

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MUK78_RS03315 Protein ID WP_000550189.1
Coordinates 672537..672851 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MUK78_RS03320 Protein ID WP_000560266.1
Coordinates 672848..673264 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK78_RS03295 (668695) 668695..669681 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
MUK78_RS03300 (669760) 669760..670452 - 693 WP_096037754.1 vancomycin high temperature exclusion protein -
MUK78_RS03305 (670529) 670529..671032 - 504 WP_001300832.1 M48 family metallopeptidase -
MUK78_RS03310 (671117) 671117..672253 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MUK78_RS03315 (672537) 672537..672851 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MUK78_RS03320 (672848) 672848..673264 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MUK78_RS03325 (673309) 673309..675327 - 2019 WP_113396716.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
MUK78_RS03330 (675753) 675753..678104 - 2352 WP_000695495.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T241273 WP_000550189.1 NZ_CP095088:672537-672851 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T241273 NZ_LT969519:113016-113540 [Pseudomonas aeruginosa]
ATGAAAATCATCGGAGTGCAGGACCTCCCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACCCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTTGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGGAAGCGCATTCCTTCCACCAGGTCTGCTGGTCAGCTATTCCCTCAGTCTGGACCGAGTGATC
GACTTCACCGGCGGCTATGACGATACCTGGGCCCCGATCTGGCAAGACTTTTTCTGCGACTGGAGAAAGATTCGCTTCAA
CGACGGCGTTGAACCGCCGAGCTGGATTGTCGGGGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTAAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGACGCGCCCGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT241273 WP_000560266.1 NZ_CP095088:672848-673264 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT241273 NZ_LT969519:112624-113019 [Pseudomonas aeruginosa]
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCTCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACCGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAAGCCTACATTCGTGATTCAGTGAAGGTTCTGCGTGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCAACTACAAGACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCCTGGGAAGCTGGTGCGCAAGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References