241272

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 629204..630003 Replicon chromosome
Accession NZ_CP095088
Organism Escherichia coli isolate w264

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag MUK78_RS03090 Protein ID WP_000347273.1
Coordinates 629204..629668 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MUK78_RS03095 Protein ID WP_001307405.1
Coordinates 629668..630003 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK78_RS03060 (624205) 624205..624639 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MUK78_RS03065 (624657) 624657..625535 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
MUK78_RS03070 (625525) 625525..626304 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MUK78_RS03075 (626315) 626315..626788 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MUK78_RS03080 (626811) 626811..628091 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
MUK78_RS03085 (628340) 628340..629149 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MUK78_RS03090 (629204) 629204..629668 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MUK78_RS03095 (629668) 629668..630003 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MUK78_RS03100 (630152) 630152..631723 - 1572 WP_001723936.1 galactarate dehydratase -
MUK78_RS03105 (632098) 632098..633432 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
MUK78_RS03110 (633448) 633448..634218 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T241272 WP_000347273.1 NZ_CP095088:c629668-629204 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T241272 NZ_LT963436:c2437653-2437558 [Staphylococcus saprophyticus]
ATGCTTATGATCTTCGTTCACATCATTGCACCAGTCATTAGTGGCTGTGCAGTTGCGTATTTTACTTATTGGCTTAGTAG
TAAACGCAATAAATAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT241272 WP_001307405.1 NZ_CP095088:c630003-629668 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT241272 NZ_LT963436:2437496-2437529 [Staphylococcus saprophyticus]
CACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References