Detailed information of TA system TA241258

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4303996..4304405
• Replicon: chromosome
• Accession: NZ_CP095086
• Organism: Escherichia coli isolate EC5654

Toxin (Protein)

• Gene name: symE
• Uniprot ID: -
• Locus tag: MUK77_RS20695
• Protein ID: WP_001534835.1
• Coordinates: 4304067..4304405 (+)
• Length: 113 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4303996..4304072 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK77_RS20685 (4299184)	4299184..4300494	+	1311	WP_001534837.1	restriction endonuclease subunit S	-
MUK77_RS20690 (4300589)	4300589..4303852	+	3264	WP_001534836.1	HsdR family type I site-specific deoxyribonuclease	-
- (4303996)	4303996..4304072	-	77	NuclAT_6	-	Antitoxin
- (4303996)	4303996..4304072	-	77	NuclAT_6	-	Antitoxin
- (4303996)	4303996..4304072	-	77	NuclAT_6	-	Antitoxin
- (4303996)	4303996..4304072	-	77	NuclAT_6	-	Antitoxin
- (4303996)	4303996..4304072	-	77	NuclAT_7	-	Antitoxin
- (4303996)	4303996..4304072	-	77	NuclAT_7	-	Antitoxin
- (4303996)	4303996..4304072	-	77	NuclAT_7	-	Antitoxin
- (4303996)	4303996..4304072	-	77	NuclAT_7	-	Antitoxin
MUK77_RS20695 (4304067)	4304067..4304405	+	339	WP_001534835.1	endoribonuclease SymE	Toxin
MUK77_RS20700 (4304493)	4304493..4304627	-	135	WP_001314403.1	hypothetical protein	-
MUK77_RS20705 (4304789)	4304789..4305097	+	309	Protein_4066	winged helix-turn-helix domain-containing protein	-
MUK77_RS20710 (4305089)	4305089..4306009	-	921	WP_000181193.1	Rpn family recombination-promoting nuclease/putative transposase	-
MUK77_RS20715 (4306194)	4306194..4307474	+	1281	WP_016238255.1	DUF445 domain-containing protein	-
MUK77_RS20720 (4307590)	4307590..4308741	+	1152	WP_001338076.1	double-cubane-cluster-containing anaerobic reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12310.04 Da
• Isoelectric Point: 7.8226

>T241258 WP_001534835.1 NZ_CP095086:4304067-4304405 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYNRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQDFIGVISNKTPR

Nucleotide

• Length: 339 bp

>T241258 NZ_LT963408:c6093700-6093410 [Pseudomonas syringae group genomosp. 3]
ATGACTTATAGCCTCGAATTTGATGCGCGAGCTCTGAAGGAATGGCACAAATTAGGGGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGTGTGTTGAAGCAAATCGCCTGCATGCGTTGCCCGACTGCTACAAAATCA
AGCTGCGCAGCAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCTGTGGACAAG
CGTGAGCGAGATGAGGTTTACCGTAAGGCGGCTGATCGGTTGGGCGGGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT241258 NZ_CP095086:c4304072-4303996 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin