Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2574611..2575173 Replicon chromosome
Accession NZ_CP095086
Organism Escherichia coli isolate EC5654

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag MUK77_RS12255 Protein ID WP_000605675.1
Coordinates 2574611..2574889 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MUK77_RS12260 Protein ID WP_000781370.1
Coordinates 2574889..2575173 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK77_RS12230 (2570199) 2570199..2570630 - 432 WP_000152305.1 peroxiredoxin OsmC -
MUK77_RS12235 (2570975) 2570975..2571190 + 216 WP_000495766.1 biofilm-dependent modulation protein -
MUK77_RS12240 (2571292) 2571292..2571429 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MUK77_RS12245 (2571585) 2571585..2573282 + 1698 WP_000433462.1 malate dehydrogenase -
MUK77_RS12250 (2573415) 2573415..2574425 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
MUK77_RS12255 (2574611) 2574611..2574889 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUK77_RS12260 (2574889) 2574889..2575173 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MUK77_RS12265 (2575402) 2575402..2576055 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
MUK77_RS12270 (2576048) 2576048..2576932 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
MUK77_RS12275 (2576945) 2576945..2579992 - 3048 WP_143355451.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T241250 WP_000605675.1 NZ_CP095086:2574611-2574889 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T241250 NZ_LT963406:c59743-59459 [Pseudomonas syringae pv. avii]
ATGAAAGAACAAATTTTCGATTACGACCCAGCAGCAGCCCTTGACGGGCCTGAGGCAATCGCTATTTTTATCTCTGACGC
ATTTGAGACGGGGGATGCGGAGTACATCGCGAAGGCTATGGGGGTTGTCGCAAGGGCCAAAGGGATGAGCGAGCTTGCGC
GTGAGACAGGTCTTTCTCGAGAGCAGCTTTACAGATCGTTCAGTAGCAAGGGCAATCCAACCTTAAAAACCATGTTGACG
GTAATGAGAGCCCTGGGCGTGGACATGACAGCTCGAATGCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT241250 WP_000781370.1 NZ_CP095086:2574889-2575173 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT241250 NZ_LT963406:c60039-59740 [Pseudomonas syringae pv. avii]
ATGACGACGATAAAACAGACATCTACCTATATGGCTTGGGAACGCAGGCTCAAAGACCAAAAGGCCAAAGCTGCAATTGC
CGCTCGTATTTTTAGAGTTGCAAATGGCCTGATGGGTGATGTCTCGCCAGTAGGGCAGGGCGTGAGTGAGCTACGGATAC
ACGTTGGCCCTGGTTACCGTGTTTATTTCCAGCAGCGAGGCGATGAACTGATCTTGCTGCTGTGCGGTGGTGACAAGAGC
AGCCAATCCCGAGATATCGAAACAGCAAAGAGACTGGCTGATCAATGGAGGCAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References