Detailed information of TA system TA241238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1213849..1214576
• Replicon: chromosome
• Accession: NZ_CP095086
• Organism: Escherichia coli isolate EC5654

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: MUK77_RS05835
• Protein ID: WP_000547563.1
• Coordinates: 1213849..1214160 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MUK77_RS05840
• Protein ID: WP_000126304.1
• Coordinates: 1214157..1214576 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK77_RS05805 (1208993)	1208993..1210702	+	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-
MUK77_RS05810 (1210712)	1210712..1211254	+	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
MUK77_RS05815 (1211254)	1211254..1212021	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
MUK77_RS05820 (1212018)	1212018..1212428	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
MUK77_RS05825 (1212421)	1212421..1212888	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
MUK77_RS05830 (1212931)	1212931..1213686	+	756	WP_001314074.1	hypothetical protein	-
MUK77_RS05835 (1213849)	1213849..1214160	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
MUK77_RS05840 (1214157)	1214157..1214576	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
MUK77_RS05845 (1214668)	1214668..1215096	-	429	WP_000536066.1	DUF4259 domain-containing protein	-
MUK77_RS05850 (1215481)	1215481..1216008	+	528	WP_001078777.1	electron transport protein HydN	-
MUK77_RS05855 (1216161)	1216161..1218413	+	2253	WP_001535518.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1185003..1223921	38918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T241238 WP_000547563.1 NZ_CP095086:1213849-1214160 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T241238 NZ_LT963402:c273251-272976 [Pseudomonas syringae pv. avii]
ATGGAATTGAAGTGGACAAGCAAGGCGCTTTCCGACATTACCCGGCTATACGAGTTTCTGGCCTCTGTGAATCAACCTGC
TGCCGCACGAGCAGTGCAGCAACTCACCACCGCACCTACCACGCTGCTGACCAATCCGCGCATTGGAGAACGCCTGGAGG
AATTCGAGCCAAGGGACGTACGCCGAATTCAGATCGGCCAATACGAGATGCGTTACGAGATAGCGGATTCCACTATCTAC
CTGCTGCGCCTGTGGCACACCCGCGAAGACCGATAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT241238 WP_000126304.1 NZ_CP095086:1214157-1214576 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT241238 NZ_LT963402:c273523-273251 [Pseudomonas syringae pv. avii]
ATGGCAACCACCGCCAAAACCCGATCCGTCACAGCTCACGTTCCGGTGCAACTGGCGGAAAAAGTCGATTTGATGGCTGA
GCGTCTGGAGCGCTCCAAGAACTGGATCGTCAAGCAAGCTCTGTCCGCTTGGATAGACCAAGAAGAAGAGCGTAGTCGCC
TGACCCGTGAGGCTTTGACTGATGTTGACGCTGGACGCGTTATTGACCACCAAGCCGTCCAGGCCTGGGCTGACAGTCTC
AGCACCGACAATCCGTTGCCGGTGCCGCGCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22