Detailed information of TA system TA241233

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25634..26056
• Replicon: plasmid pEC6868-1
• Accession: NZ_CP095084
• Organism: Escherichia coli strain EC6868

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MUK76_RS25775
• Protein ID: WP_096937776.1
• Coordinates: 25931..26056 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	25634..25833 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK76_RS25740 (20829)	20829..21281	-	453	WP_024185774.1	hypothetical protein	-
MUK76_RS25745 (21583)	21583..22110	+	528	WP_000290833.1	single-stranded DNA-binding protein	-
MUK76_RS25750 (22167)	22167..22400	+	234	WP_000006018.1	DUF905 domain-containing protein	-
MUK76_RS25755 (22459)	22459..24420	+	1962	WP_244750114.1	ParB/RepB/Spo0J family partition protein	-
MUK76_RS25760 (24475)	24475..24909	+	435	WP_000845910.1	conjugation system SOS inhibitor PsiB	-
MUK76_RS25765 (24906)	24906..25666	+	761	Protein_33	plasmid SOS inhibition protein A	-
- (25634)	25634..25833	+	200	NuclAT_0	-	Antitoxin
- (25634)	25634..25833	+	200	NuclAT_0	-	Antitoxin
- (25634)	25634..25833	+	200	NuclAT_0	-	Antitoxin
- (25634)	25634..25833	+	200	NuclAT_0	-	Antitoxin
MUK76_RS25770 (25840)	25840..25989	+	150	Protein_34	DUF5431 family protein	-
MUK76_RS25775 (25931)	25931..26056	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
MUK76_RS25780 (26373)	26373..26670	-	298	Protein_36	hypothetical protein	-
MUK76_RS25785 (26971)	26971..27258	+	288	WP_000107526.1	hypothetical protein	-
MUK76_RS25790 (27377)	27377..28199	+	823	Protein_38	DUF932 domain-containing protein	-
MUK76_RS25795 (28493)	28493..29001	-	509	Protein_39	transglycosylase SLT domain-containing protein	-
MUK76_RS25800 (29423)	29423..29806	+	384	WP_001151562.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MUK76_RS25805 (29999)	29999..30683	+	685	Protein_41	PAS domain-containing protein	-
MUK76_RS25810 (30777)	30777..31004	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5	senB	1..144934	144934

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T241233 WP_096937776.1 NZ_CP095084:25931-26056 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T241233 NZ_LT963402:181751-182029 [Pseudomonas syringae pv. avii]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGCTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGCGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCCATTCAGGATTTGCTTCGTTTGGAGCATC
AACGGTCCCGAGGACGTAGAAATTGTCGATTATCACTGA

Antitoxin

Nucleotide

• Length: 200 bp

>AT241233 NZ_CP095084:25634-25833 [Escherichia coli]
TCACAACGGATTGCCCGTAAACAGCCTGAATGAGCGAGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATAT
GGGCTATGATGTGCCCGGCGCTTGAGGTTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGG
ACAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin