Detailed information of TA system TA241227

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 4484504..4484916
• Replicon: chromosome
• Accession: NZ_CP095083
• Organism: Escherichia coli strain EC6868

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: MUK76_RS21930
• Protein ID: WP_000132601.1
• Coordinates: 4484575..4484916 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4484504..4484580 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK76_RS21920 (4481367)	4481367..4482956	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
MUK76_RS21925 (4482953)	4482953..4484347	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
- (4484504)	4484504..4484580	-	77	NuclAT_12	-	Antitoxin
- (4484504)	4484504..4484580	-	77	NuclAT_12	-	Antitoxin
- (4484504)	4484504..4484580	-	77	NuclAT_12	-	Antitoxin
- (4484504)	4484504..4484580	-	77	NuclAT_12	-	Antitoxin
- (4484504)	4484504..4484580	-	77	NuclAT_13	-	Antitoxin
- (4484504)	4484504..4484580	-	77	NuclAT_13	-	Antitoxin
- (4484504)	4484504..4484580	-	77	NuclAT_13	-	Antitoxin
- (4484504)	4484504..4484580	-	77	NuclAT_13	-	Antitoxin
MUK76_RS21930 (4484575)	4484575..4484916	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
MUK76_RS21935 (4485078)	4485078..4486457	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
MUK76_RS21940 (4486457)	4486457..4487503	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T241227 WP_000132601.1 NZ_CP095083:4484575-4484916 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T241227 NZ_LT963398:10313-10663 [Pseudomonas cerasi]
ATGAATACGTTTGCACTACGCTTTACCGAGGTTGCCCAGCAAAGCATTGAAGATCAGGTCGAGCACCTGGCTATCACTCA
GGGATTCTCATCAGCTGCTCAGCGCATAGATACCCTGATCGATGCCATCCAGGACAAGCTCCTGTCCACCCCTTTGGGCT
ACCCGGTCAGTCCACAGTTGAGTGAGCTGGGTGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGGATCTTTTAT
GAGGTGATGGATTCTGATGGTATTACCGATATCGCCGTATTGCTCGTATTAGGCGGGAAACAAAGTGTGGAGCAGGCTCT
GATCCGCTACTGCCTGCTCCAGCCGATGTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT241227 NZ_CP095083:c4484580-4484504 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin