Detailed information of TA system TA241224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4093206..4093856
• Replicon: chromosome
• Accession: NZ_CP095083
• Organism: Escherichia coli strain EC6868

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MUK76_RS20110
• Protein ID: WP_000263532.1
• Coordinates: 4093515..4093856 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: MUK76_RS20105
• Protein ID: WP_000212552.1
• Coordinates: 4093206..4093505 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK76_RS20080 (4088654)	4088654..4088965	-	312	WP_000415004.1	hypothetical protein	-
MUK76_RS20085 (4088970)	4088970..4089362	-	393	WP_000285322.1	flagellar export chaperone FliS	-
MUK76_RS20090 (4089385)	4089385..4090701	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
MUK76_RS20095 (4090906)	4090906..4091820	-	915	WP_000949083.1	lateral flagellin LafA	-
MUK76_RS20100 (4092306)	4092306..4093148	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
MUK76_RS20105 (4093206)	4093206..4093505	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
MUK76_RS20110 (4093515)	4093515..4093856	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MUK76_RS20115 (4093932)	4093932..4094909	-	978	WP_001195938.1	hypothetical protein	-
MUK76_RS20120 (4094926)	4094926..4095855	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
MUK76_RS20125 (4095870)	4095870..4097246	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
MUK76_RS20130 (4097422)	4097422..4097721	-	300	WP_000867281.1	rod-binding protein	-
MUK76_RS20135 (4097721)	4097721..4098821	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4049594..4093859	44265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T241224 WP_000263532.1 NZ_CP095083:c4093856-4093515 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T241224 NZ_LT963395:c4196374-4196192 [Pseudomonas cerasi]
GTGCAGAGCAGGCAATTGATAAAGGAGCTTGAAGCAGATGGATGGATTCTGGATCGTGTTTCAGGCAGTCACCATATGTT
CAAGCATCCTGAAAAACTTCAGACAGTCCCCGTACCGCATCCCAAGAAAGACTTGCCATTCGGAACGGTGCGGGCCATCA
AAAAGCTTGCCGGGCTGGTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT241224 WP_000212552.1 NZ_CP095083:c4093505-4093206 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT241224 NZ_LT963395:c4196156-4195752 [Pseudomonas cerasi]
ATGAAATATCCAATGTGCATAGAATGGGGAAGCGAAACCACGGCGTTTGGTATCCAGATTCCCGATATCCCAGGTGCCGT
AACCGCAGGCGATACGTTCGAGGAAGCCCACGCCGCGGCGGTGGAGATTGCTCATATCATGCTTCAGGAAATAGCAGCTG
GTGGTGGAACCATACCCAAGGCCGGCACTGTTGCCGAGCACGCCAGGAATGCCGATTTCGCCGGAATGGGCTGGGGCATG
ATCGAGATTGACGTAACGCCTTATCTTGGTAAGACGGAGAAGGTCAACGTCACACTGCCTGGTTTCGTGATCAAGCAGAT
CGATCGCTATGTACGGGATCACAGCATCAAGAGTCGTTCCACCTTCCTGGCCGACGCGGCGCTGGAAAAACTGGGCCGGG
CATGA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7