Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2606520..2607082 Replicon chromosome
Accession NZ_CP095083
Organism Escherichia coli strain EC6868

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag MUK76_RS12490 Protein ID WP_000605676.1
Coordinates 2606520..2606798 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MUK76_RS12495 Protein ID WP_000781370.1
Coordinates 2606798..2607082 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK76_RS12465 (2602106) 2602106..2602537 - 432 WP_000152305.1 peroxiredoxin OsmC -
MUK76_RS12470 (2602882) 2602882..2603097 + 216 WP_116842948.1 biofilm-dependent modulation protein -
MUK76_RS12475 (2603199) 2603199..2603336 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MUK76_RS12480 (2603493) 2603493..2605190 + 1698 WP_000433464.1 malate dehydrogenase -
MUK76_RS12485 (2605324) 2605324..2606334 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
MUK76_RS12490 (2606520) 2606520..2606798 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUK76_RS12495 (2606798) 2606798..2607082 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MUK76_RS12500 (2607310) 2607310..2607963 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
MUK76_RS12505 (2607956) 2607956..2608840 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
MUK76_RS12510 (2608853) 2608853..2611900 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T241219 WP_000605676.1 NZ_CP095083:2606520-2606798 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T241219 NZ_LT963391:c433776-433501 [Pseudomonas syringae pv. cerasicola]
ATGGAATTGAAGTGGACAAGCAAGGCGCTTTCCGACATTACTCGACTGTACGAGTTTCTGGCCTCGGTGAATCAGCCTGC
CGCCGCACGAACAGTGCAGCAACTCACAGCCGCACCTACCACGCTGCTGACCAACCCGCGTATTGGCGAACGGCTGGAAG
AGTTCGAGCCAAGGGATGTACGCCGAATTCAGATCGGCCAATACGAGATGCGTTACGAGCTAGTTGATTCCACTATCTAC
CTGCTGCGCCTGTGGCACACCCGCGAAGACCGATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT241219 WP_000781370.1 NZ_CP095083:2606798-2607082 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT241219 NZ_LT963391:c434048-433776 [Pseudomonas syringae pv. cerasicola]
ATGGCAACCACCGCGAAAACCCGATCTGTCACGGCTCACGTCCCTGAGCAACTGGCCCAAAAGGTCGATTTGATGGCAGA
GCGTCTGGAGCGCTCCAAAAACTGGATCGTCAAGCAGGCTCTGTCCGCTTGGATAGACCAGGAAGAAGAGCGTAGTCGCC
TGACCCGTGAGGCCTTGGCCGATGTTGACGCTGGCCGCGTTATTGACCACCAAGCCGTCCAGGCCTGGGCTGACAGCCTC
AGCACCGACACTCCGTTGCCGGTGCCGGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References