Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 641300..642099 Replicon chromosome
Accession NZ_CP095083
Organism Escherichia coli strain EC6868

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag MUK76_RS03105 Protein ID WP_000347273.1
Coordinates 641300..641764 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MUK76_RS03110 Protein ID WP_001307405.1
Coordinates 641764..642099 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK76_RS03075 (636301) 636301..636735 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MUK76_RS03080 (636753) 636753..637631 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MUK76_RS03085 (637621) 637621..638400 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
MUK76_RS03090 (638411) 638411..638884 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
MUK76_RS03095 (638907) 638907..640187 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
MUK76_RS03100 (640436) 640436..641245 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MUK76_RS03105 (641300) 641300..641764 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MUK76_RS03110 (641764) 641764..642099 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MUK76_RS03115 (642248) 642248..643819 - 1572 WP_001273753.1 galactarate dehydratase -
MUK76_RS03120 (644194) 644194..645528 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MUK76_RS03125 (645544) 645544..646314 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T241205 WP_000347273.1 NZ_CP095083:c641764-641300 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T241205 NZ_LT962930:1044668-1044913 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT241205 WP_001307405.1 NZ_CP095083:c642099-641764 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT241205 NZ_LT962930:1044913-1045227 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References