Detailed information of TA system TA241185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3548911..3549581
• Replicon: chromosome
• Accession: NZ_CP095023
• Organism: Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MUW68_RS16790
• Protein ID: WP_244732990.1
• Coordinates: 3548911..3549255 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: MUW68_RS16795
• Protein ID: WP_003899955.1
• Coordinates: 3549252..3549581 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW68_RS16760 (MUW68_16760)	3543991..3544202	+	212	Protein_3313	(R)-hydratase	-
MUW68_RS16765 (MUW68_16765)	3544215..3544508	+	294	WP_003416635.1	hypothetical protein	-
MUW68_RS16770 (MUW68_16770)	3544796..3546085	+	1290	WP_003416640.1	ATP-binding protein	-
MUW68_RS16780 (MUW68_16780)	3547791..3548225	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
MUW68_RS16785 (MUW68_16785)	3548228..3548680	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MUW68_RS16790 (MUW68_16790)	3548911..3549255	+	345	WP_244732990.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MUW68_RS16795 (MUW68_16795)	3549252..3549581	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
MUW68_RS16800 (MUW68_16800)	3550119..3550466	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
MUW68_RS16805 (MUW68_16805)	3550463..3551083	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
MUW68_RS16810 (MUW68_16810)	3551243..3552508	-	1266	WP_003902423.1	hypothetical protein	-
MUW68_RS16815 (MUW68_16815)	3552676..3552885	+	210	WP_003416778.1	hypothetical protein	-
MUW68_RS16820 (MUW68_16820)	3553132..3554166	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12678.47 Da
• Isoelectric Point: 5.6845

>T241185 WP_244732990.1 NZ_CP095023:3548911-3549255 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLDMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T241185 NZ_LT960615:c106997-106608 [Hartmannibacter diazotrophicus]
ATGGTAATCGACACATCGGCAATTGTTGCAATTTTCTTCAATGAGCCGGATGCGATGACATATCGCGAACGCATCGCTGC
CGACCCGATACGCTTGATTTCGGCGGCAACGCTGCTGGAAGCTTCGATGGTCATCGAGGGCCGGTTTGGTGAAGCGGGGG
GTGCGGAATTCGATCTTTGGCTGCATAAGGCCGAGGTAGAGGTCGTTGCGGTAAACCATGAACACGCCGACCAGGCGCGG
CGAGCATGGCGCCGCTACGGAAAGGGCCGTCATCCGGCGAGCCTGAATTATGGAGACTGCTTTTCCTACGCTCTTTCGAG
CCTGTCCGGCGAGCCCCTCCTTTTCAAGGGCAACGACTTCAAACAGACAGATATCCAGGCGGTATCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT241185 WP_003899955.1 NZ_CP095023:3549252-3549581 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT241185 NZ_LT960615:c107251-106997 [Hartmannibacter diazotrophicus]
ATGGCCCTCAGCATCAAAGATCCCGAAACTGAAAAACTCGCACGCGCGCTGGCGGTGCGGACCGGCGAAACCATCACGGT
TGCGACACGGCGCGCTCTCGAGGAGCGCCTCAGGCGTATCGGGTTGGATGCGAAAAAGGCTGCTCTGATCGAGGACATGG
AGGCCATGCAACGTCGCCTGAGCGTCCTGCCGGTGCTCGACGATCGCAGCGCCGATGACATTATCGGCTACGACGAAAAC
GGAATTCCGATCTGA

Structures

Antitoxin

• PDB: 6LTY
• PDB: 6LTZ
• AlphaFold DB: G0THF6