Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2262573..2263492 Replicon chromosome
Accession NZ_CP095023
Organism Mycobacterium tuberculosis strain SGF0702019

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag MUW68_RS10625 Protein ID WP_003900449.1
Coordinates 2262887..2263492 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag MUW68_RS10620 Protein ID WP_003410124.1
Coordinates 2262573..2262878 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW68_RS10585 (MUW68_10585) 2257836..2258036 + 201 Protein_2094 hypothetical protein -
MUW68_RS10595 (MUW68_10595) 2259392..2259769 + 378 Protein_2096 hypothetical protein -
MUW68_RS10600 (MUW68_10600) 2259766..2260806 + 1041 WP_003918360.1 ImmA/IrrE family metallo-endopeptidase -
MUW68_RS10605 (MUW68_10605) 2261048..2261767 + 720 WP_003410108.1 DUF433 domain-containing protein -
MUW68_RS10610 (MUW68_10610) 2261757..2262173 + 417 WP_003410114.1 hypothetical protein -
MUW68_RS10615 (MUW68_10615) 2262189..2262488 - 300 WP_003410120.1 hypothetical protein -
MUW68_RS10620 (MUW68_10620) 2262573..2262878 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
MUW68_RS10625 (MUW68_10625) 2262887..2263492 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUW68_RS10630 (MUW68_10630) 2263517..2263876 - 360 WP_003410131.1 hypothetical protein -
MUW68_RS10635 (MUW68_10635) 2264036..2264626 - 591 WP_223695536.1 hypothetical protein -
MUW68_RS10640 (MUW68_10640) 2264918..2266179 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
MUW68_RS10645 (MUW68_10645) 2266466..2267362 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2258080..2273835 15755


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T241167 WP_003900449.1 NZ_CP095023:c2263492-2262887 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T241167 NZ_LT934425:2310143-2310544 [Candidatus Kuenenia stuttgartiensis]
ATGAAATGTATGCTTGATACCAATATATGCATTTATCTCATCAAGCGGAAAAACCCTAAAATCCTTGCGTATCTCAAAAA
ACACGACATAGGGGAGGTTGGTATTTCATCTATTACCCTCGCGGAACTTCAATACGGGGTGGCAAACAGCAATTATATCC
AGAGAAACAGAGAGGCTTTACATGAGTTTATCCTGCCTCTTGAAATTGCAGATTTCGATGAAAAGGTTGCTGAGGTGTAC
GGGAATGTGAGGGCGAACCTTGAAAAGGCAGGAACCCCTATTGGCTCAATGGATATGCTCATTGGCGCTCATGCCATGAG
TCTCGGTGTAACCCTTGTATCTAATAATACCAGGGAATTTAATCGTATAAAAGGGCTTAAGGTTGTAGACTGGACCATTT
GA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT241167 WP_003410124.1 NZ_CP095023:c2262878-2262573 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT241167 NZ_LT934425:2309916-2310143 [Candidatus Kuenenia stuttgartiensis]
GTGAAAACTGCAAAAATTTTTCAAAACGGTCAAAGCCAGGCTGTTAGGCTGCCCAAGGAGTTTCGGTTTGAGGGTGACCA
CGTATTTATCAAAAAGTCGGGAAATATTGTGATGCTTATTCCAGCCAAAGAATCGTGGAATTCTTTGGTAGACAGCCTTG
ATAAATTTACCGATGACTTCATGGGTGAGAGAAAACAGTTAGATACCCAGACAAGGGAGCCACTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References