Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3548717..3549387 Replicon chromosome
Accession NZ_CP095022
Organism Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag MUW67_RS16830 Protein ID WP_003899954.1
Coordinates 3548717..3549061 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag MUW67_RS16835 Protein ID WP_003899955.1
Coordinates 3549058..3549387 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW67_RS16800 (MUW67_16800) 3543798..3544009 + 212 Protein_3316 (R)-hydratase -
MUW67_RS16805 (MUW67_16805) 3544022..3544315 + 294 WP_244753770.1 hypothetical protein -
MUW67_RS16810 (MUW67_16810) 3544603..3545892 + 1290 WP_003416640.1 ATP-binding protein -
MUW67_RS16820 (MUW67_16820) 3547597..3548031 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
MUW67_RS16825 (MUW67_16825) 3548034..3548486 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MUW67_RS16830 (MUW67_16830) 3548717..3549061 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUW67_RS16835 (MUW67_16835) 3549058..3549387 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
MUW67_RS16840 (MUW67_16840) 3549925..3550272 + 348 WP_003899956.1 DUF2384 domain-containing protein -
MUW67_RS16845 (MUW67_16845) 3550269..3550889 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
MUW67_RS16850 (MUW67_16850) 3551049..3552314 - 1266 WP_003902423.1 hypothetical protein -
MUW67_RS16855 (MUW67_16855) 3552482..3552691 + 210 WP_003416778.1 hypothetical protein -
MUW67_RS16860 (MUW67_16860) 3552938..3553972 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T241125 WP_003899954.1 NZ_CP095022:3548717-3549061 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T241125 NZ_LT906615:c399583-399293 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT241125 WP_003899955.1 NZ_CP095022:3549058-3549387 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT241125 NZ_LT906615:c399821-399573 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References