Detailed information of TA system TA024111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 256568..257262
• Replicon: chromosome
• Accession: NC_012967
• Organism: Escherichia coli B str. REL606

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: ECB_RS01200
• Protein ID: WP_001263493.1
• Coordinates: 256864..257262 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ECB_RS01195
• Protein ID: WP_000554757.1
• Coordinates: 256568..256861 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECB_RS01175	252208..252705	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
ECB_RS01180	252921..254633	-	1713	Protein_224	flagellar biosynthesis protein FlhA	-
ECB_RS01185	254605..255390	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
ECB_RS01190	255461..256516	+	1056	WP_001226164.1	DNA polymerase IV	-
ECB_RS01195	256568..256861	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ECB_RS01200	256864..257262	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ECB_RS01205	257272..257724	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
ECB_RS01210	257970..258173	+	204	Protein_230	RNA ligase RtcB family protein	-
ECB_RS01215	258172..258693	+	522	Protein_231	peptide chain release factor H	-
ECB_RS01220	258750..260207	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
ECB_RS01225	260468..260926	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ECB_RS01230	261018..262262	+	1245	WP_000189541.1	esterase FrsA	-
-	261522..261602	+	81	NuclAT_13	-	-
-	261522..261602	+	81	NuclAT_13	-	-
-	261522..261602	+	81	NuclAT_13	-	-
-	261522..261602	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T24111 WP_001263493.1 NC_012967:256864-257262 [Escherichia coli B str. REL606]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T24111 NC_012967:256864-257262 [Escherichia coli B str. REL606]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT24111 WP_000554757.1 NC_012967:256568-256861 [Escherichia coli B str. REL606]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT24111 NC_012967:256568-256861 [Escherichia coli B str. REL606]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6