Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2260705..2261624 Replicon chromosome
Accession NZ_CP095022
Organism Mycobacterium tuberculosis strain SGF0472017

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag MUW67_RS10650 Protein ID WP_003900449.1
Coordinates 2261019..2261624 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag MUW67_RS10645 Protein ID WP_003410124.1
Coordinates 2260705..2261010 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW67_RS10610 (MUW67_10610) 2255968..2256168 + 201 Protein_2095 hypothetical protein -
MUW67_RS10620 (MUW67_10620) 2257524..2257901 + 378 Protein_2097 hypothetical protein -
MUW67_RS10625 (MUW67_10625) 2257898..2258938 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
MUW67_RS10630 (MUW67_10630) 2259180..2259899 + 720 WP_003410108.1 DUF433 domain-containing protein -
MUW67_RS10635 (MUW67_10635) 2259889..2260305 + 417 WP_003410114.1 hypothetical protein -
MUW67_RS10640 (MUW67_10640) 2260321..2260620 - 300 WP_003410120.1 hypothetical protein -
MUW67_RS10645 (MUW67_10645) 2260705..2261010 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
MUW67_RS10650 (MUW67_10650) 2261019..2261624 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUW67_RS10655 (MUW67_10655) 2261649..2262008 - 360 WP_003410131.1 hypothetical protein -
MUW67_RS10660 (MUW67_10660) 2262168..2262758 - 591 WP_223695536.1 hypothetical protein -
MUW67_RS10665 (MUW67_10665) 2262797..2263033 - 237 WP_003410133.1 hypothetical protein -
MUW67_RS10670 (MUW67_10670) 2263240..2264136 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2254083..2270609 16526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T241107 WP_003900449.1 NZ_CP095022:c2261624-2261019 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T241107 NZ_LT906479:c4268163-4267741 [Serratia ficaria]
GTGGCCCAGTGGCGATGTGACGTCCGCATTTCCTGGCGCACCCAGTTATTTTCGCTGCTGACGCACGGCATTCTGATACT
GCTGATCCTGATTTCGCCATGGCCGGAAGGCTACGGGCCGATCTGGCTGGTGCTGTTGACGCTGGTGGTGTTTCAATGCA
TCCGCAGCCAGAAGCGCATCGCCGCGGCGCAGGGAGAACTGCGCCTGCTGGCGGACCGGCGTTTCAGCTGGCACGGCCGC
GAATGGCGGTTGGCGAAAAAGCCCTGGATGCTCGGCTACGGCATGCTGCTGACGCTGCAGCCGCTGGCGGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCCTCGGACAGCATGGGTAAGGAAGAGTGGCGGCATCTGCGCCAGCTGTTGCTGTATCCGCCGG
CCGACGATGACGAGGACGCCTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT241107 WP_003410124.1 NZ_CP095022:c2261010-2260705 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT241107 NZ_LT906479:c4268410-4268144 [Serratia ficaria]
ATGGATATTAATAACAAAGCACGTATTCACTGGGCCTGCCGCCGCGGCATGCGCGAGTTGGACATCTCCATCATGCCGTT
CTTCGAATACGAGTACGACCAGCTGAATGATACCGACAAGGCGCTGTTTATCCGCCTGCTGGAGTGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGACAGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References