Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3540149..3540819 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag MUW66_RS16775 Protein ID WP_003899954.1
Coordinates 3540149..3540493 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag MUW66_RS16780 Protein ID WP_003899955.1
Coordinates 3540490..3540819 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS16745 (MUW66_16745) 3535230..3535441 + 212 Protein_3308 (R)-hydratase -
MUW66_RS16750 (MUW66_16750) 3535454..3535747 + 294 WP_003416635.1 hypothetical protein -
MUW66_RS16755 (MUW66_16755) 3536035..3537324 + 1290 WP_244743693.1 ATP-binding protein -
MUW66_RS16765 (MUW66_16765) 3539029..3539463 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
MUW66_RS16770 (MUW66_16770) 3539466..3539918 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MUW66_RS16775 (MUW66_16775) 3540149..3540493 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUW66_RS16780 (MUW66_16780) 3540490..3540819 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
MUW66_RS16785 (MUW66_16785) 3541357..3541704 + 348 WP_003899956.1 DUF2384 domain-containing protein -
MUW66_RS16790 (MUW66_16790) 3541701..3542321 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
MUW66_RS16795 (MUW66_16795) 3542481..3543746 - 1266 WP_003902423.1 hypothetical protein -
MUW66_RS16800 (MUW66_16800) 3543914..3544123 + 210 WP_003416778.1 hypothetical protein -
MUW66_RS16805 (MUW66_16805) 3544370..3545404 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T241065 WP_003899954.1 NZ_CP095021:3540149-3540493 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T241065 NZ_LT906470:1974000-1974176 [Veillonella rodentium]
ATGAAGGACAAGGATTTACTTAAGCTACTTCAAAAAAACGGTTGGAAGGATGTCCGGCAGCGCGGCAGTTACCACCGATT
AAAGAAAGGTGATAAAGTGGAAGTAATCACGGTTCACGGAAGAGATGTACCCGCCGGATTATTAAACGCTATTTTAAAAC
GTACAGGACTGATATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT241065 WP_003899955.1 NZ_CP095021:3540490-3540819 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT241065 NZ_LT906470:1974186-1974563 [Veillonella rodentium]
ATGAAATTTATATATCCTGCTGTTATCCATGATGATGGCGACGGTTTATGGGCTGAATTTCCCGATTTGGAATACTCCAG
CAGCACAGGGGCAACGCTGACAGAGCTATTAACAAATGCTCAGGAAGCAATGGAATTATTCATCTTAGGTGCACTTGAGG
ATGGCGGAACTCTGCCTGAAGCCACTTCCATTCGCAATTTACCGTGTACAAAATCCACCTATCCTACACTCGTGCAAACG
GATATCGATTTAGCTAAAAACAGCAGATCAGTAAAGAAAACGCTGACCATTCCGGCATGGTTGAATGAACGCGCATTAGC
CAAAAACCTTAACTTTTCACAAATTCTACAGGAGGCCCTCGTAGAAAAGACAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References