Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2260092..2261011 Replicon chromosome
Accession NZ_CP095021
Organism Mycobacterium tuberculosis strain SGF0422017

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag MUW66_RS10630 Protein ID WP_003900449.1
Coordinates 2260406..2261011 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag MUW66_RS10625 Protein ID WP_003410124.1
Coordinates 2260092..2260397 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW66_RS10590 (MUW66_10590) 2255355..2255555 + 201 Protein_2093 hypothetical protein -
MUW66_RS10600 (MUW66_10600) 2256911..2257288 + 378 Protein_2095 hypothetical protein -
MUW66_RS10605 (MUW66_10605) 2257285..2258325 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
MUW66_RS10610 (MUW66_10610) 2258567..2259286 + 720 WP_003410108.1 DUF433 domain-containing protein -
MUW66_RS10615 (MUW66_10615) 2259276..2259692 + 417 WP_003410114.1 hypothetical protein -
MUW66_RS10620 (MUW66_10620) 2259708..2260007 - 300 WP_003410120.1 hypothetical protein -
MUW66_RS10625 (MUW66_10625) 2260092..2260397 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
MUW66_RS10630 (MUW66_10630) 2260406..2261011 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUW66_RS10635 (MUW66_10635) 2261036..2261395 - 360 WP_003410131.1 hypothetical protein -
MUW66_RS10640 (MUW66_10640) 2261555..2262208 - 654 WP_057331469.1 hypothetical protein -
MUW66_RS10645 (MUW66_10645) 2262184..2262420 - 237 WP_003410133.1 hypothetical protein -
MUW66_RS10650 (MUW66_10650) 2262627..2263523 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T241047 WP_003900449.1 NZ_CP095021:c2261011-2260406 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T241047 NZ_LT906457:c82934-81639 [Legionella spiritensis]
ATGATTGGCAGTGAACATGTATTGGATGTTTATTTGGGTGATAACATTATTGCAGAACTATCGTTAGTTAACGATCAATT
ACATTGGCATTATCGCCCTGACTGGCAACAAAATGGTTATCCCGTTTCACCACACTTACCATTAGATAGAGAAATACCAC
CTTTAAATGTAACGCGGTTTCTTCGTAATTTACTTCCAGAAGGAAATGCCTTAGAAGAGCTGATTAACACGTTTCACTTG
AGCAAAGGCAATACATTTGGCTTGGTTCGCGCTCTTGGTTTAGACACGCCCGGCTCATTAGTCTTCCGATCTCCAACACA
ATCGTCCCCAATAAAAACATCTTTTCGTCCAGTCCTTGACAAAGAATTGGAGCAACGCTTGGATAGCCGAGATGAATTTA
GTTTGATTATCTGGGATGGAAAACCTCGACTTTCAGTAGCCGGTGTTCAAGATAAAATTAATGTTGTGTTAAACGAGGAA
AATCAACTTGGTTTTGGTGAAGGTAATTTGTGCTCAACACATATTCTTAAGTTTGAGAAGCAAAAATTAGCTCACTTAGT
CCTGAATGAGTATGTCACCATGCGATTAGCTCGTCTTTGTGGACTTAATGTCGCTAATGCTAAATTGCTGTGTTATGGCA
AAAATCCGGCATTACTGGTAGAGCGTTTTGATAGACATTTTATTTCCCTAACTGAAGTTAAGAGGCGTCATATGATCGAT
GGATGTCAGGCATTAAATGTCCCTCCTGATTATAAATATGAACGTAATTTTGGAAGCGGGCGGGATGTAGCGCATATTAG
AGATGGGGTGAGTTTAGAGAAACTATTTGAGTTTGCAAATCAATGCGAAAACCCAGCGCTTGCAAAGCAACGGATGTTGG
ACTGGGTGCTATTTAATACATTAATATTTAATTGCGATGCCCATGGCAAAAATATTAGCTTTTTCGTCGGATCAAAAGGC
CTGTCCTTAACACCCTTTTATGATTTAGTGAATATCAAAATGTATCCTGAATTTGAACATGACCTGGCAATGGCATTGGG
TGATGAATTTGATGAAAATAATGTTAATGCTTATCAATTAGCTGATTTTGCTGACACATGCCAATTGCCTCGCTCCTTTG
TAACGAATAGGTTGAAATACTTGGGTAAAAAATTAAGTGCCTCAGTCGGGGAATGTCATTCAGCTGGGATTAATGATGAT
GAAAGAGCATACTTACATCACTATAAGAAAATGATTCAAGAGCGTTGTAACCATCTGCTTAAAGAGGCTGATGGTATCGT
TTCAATGGAGTTGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT241047 WP_003410124.1 NZ_CP095021:c2260397-2260092 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT241047 NZ_LT906457:c81544-81368 [Legionella spiritensis]
ATGCGGCAAAAAGCCGGATTAACGCAAGAAGAGTTGGCTCAGCGAATGGGAACGCAAAAAGGCAATATTTCTCGACTGGA
GAAAGGCAGCAGTAACCCAAGCTGGAAAACATTGGAAAAATACGCACATGCCTGTGGTTTTGATATCTCCATGGCATTTA
AGCGAACGGCGTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References