Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3547876..3548546 Replicon chromosome
Accession NZ_CP095020
Organism Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag MUW65_RS16805 Protein ID WP_003899954.1
Coordinates 3547876..3548220 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag MUW65_RS16810 Protein ID WP_003899955.1
Coordinates 3548217..3548546 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUW65_RS16775 (MUW65_16775) 3542957..3543168 + 212 Protein_3314 (R)-hydratase -
MUW65_RS16780 (MUW65_16780) 3543181..3543474 + 294 WP_003416635.1 hypothetical protein -
MUW65_RS16785 (MUW65_16785) 3543762..3545051 + 1290 WP_003416640.1 ATP-binding protein -
MUW65_RS16795 (MUW65_16795) 3546756..3547190 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
MUW65_RS16800 (MUW65_16800) 3547193..3547645 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MUW65_RS16805 (MUW65_16805) 3547876..3548220 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUW65_RS16810 (MUW65_16810) 3548217..3548546 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
MUW65_RS16815 (MUW65_16815) 3549084..3549431 + 348 WP_003899956.1 DUF2384 domain-containing protein -
MUW65_RS16820 (MUW65_16820) 3549428..3550048 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
MUW65_RS16825 (MUW65_16825) 3550208..3551473 - 1266 WP_003902423.1 hypothetical protein -
MUW65_RS16830 (MUW65_16830) 3551641..3551850 + 210 WP_003416778.1 hypothetical protein -
MUW65_RS16840 (MUW65_16840) 3552393..3552857 + 465 WP_003913440.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T241005 WP_003899954.1 NZ_CP095020:3547876-3548220 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T241005 NZ_LT905060:c4022040-4021756 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT241005 WP_003899955.1 NZ_CP095020:3548217-3548546 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT241005 NZ_LT905060:c4022272-4022030 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References