Detailed information of TA system TA024100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 2425533..2426125
• Replicon: chromosome
• Accession: NC_012962
• Organism: Photorhabdus asymbiotica

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PAU_RS10420
• Protein ID: WP_015834296.1
• Coordinates: 2425533..2425811 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PAU_RS10425
• Protein ID: WP_015834297.1
• Coordinates: 2425811..2426125 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAU_RS10405	2420720..2422270	-	1551	WP_015834292.1	YdgA family protein	-
PAU_RS10410	2422421..2423593	-	1173	WP_015834293.1	mannose-6-phosphate isomerase	-
PAU_RS10415	2423997..2425391	+	1395	WP_015834295.1	class II fumarate hydratase	-
PAU_RS10420	2425533..2425811	+	279	WP_015834296.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PAU_RS10425	2425811..2426125	+	315	WP_015834297.1	HigA family addiction module antidote protein	Antitoxin
PAU_RS10430	2426251..2426730	-	480	WP_015834298.1	RES family NAD+ phosphorylase	-
PAU_RS10435	2426727..2427176	-	450	WP_015834299.1	type II toxin-antitoxin system antitoxin Xre	-
PAU_RS10440	2427441..2428010	+	570	WP_015834300.1	hypothetical protein	-
PAU_RS10445	2428147..2428518	+	372	WP_015834301.1	VOC family protein	-
PAU_RS10450	2428799..2429725	-	927	WP_015834303.1	DNA replication terminus site-binding protein	-
PAU_RS10455	2429955..2431109	+	1155	WP_015834304.1	radical SAM protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-67)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10756.20 Da
• Isoelectric Point: 8.4392

>T24100 WP_015834296.1 NC_012962:2425533-2425811 [Photorhabdus asymbiotica]
MIKSFKHKGLKQLFEKGITSGVLAQDAERINDRLQAIDAANEIGELNRQIYKLHPLKGDREGYWSITVRANWRITFQFIN
GDAYILNYEDYH

Nucleotide

• Length: 279 bp

>T24100 NC_012962:2425533-2425811 [Photorhabdus asymbiotica]
ATGATTAAATCATTCAAGCACAAAGGGTTGAAGCAACTTTTTGAAAAAGGGATTACTTCTGGTGTACTGGCGCAGGATGC
CGAGAGGATTAACGACCGTTTACAGGCTATTGATGCGGCAAATGAAATAGGTGAGTTAAACCGTCAAATCTATAAACTTC
ACCCGTTGAAAGGAGATCGCGAAGGTTACTGGTCTATTACTGTCCGGGCTAACTGGCGCATTACTTTTCAGTTCATTAAC
GGTGATGCTTACATTTTGAATTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11511.20 Da
• Isoelectric Point: 7.0108

>AT24100 WP_015834297.1 NC_012962:2425811-2426125 [Photorhabdus asymbiotica]
MRQFKISHPGEIIARDLEDMGVSGRRFALNIGVTPATVSRFLAGKTALTPPLAIRIAAALGSTPEFWLRLQSAYDLRQLE
NQIDTSGIVLYGEQEEMSQVTSKY

Nucleotide

• Length: 315 bp

>AT24100 NC_012962:2425811-2426125 [Photorhabdus asymbiotica]
ATGAGACAATTTAAAATTTCCCATCCTGGAGAGATTATAGCCAGAGATTTAGAGGACATGGGTGTTAGTGGTCGTCGCTT
TGCACTCAATATTGGAGTAACACCCGCTACGGTATCGCGTTTTCTTGCCGGTAAAACTGCGTTAACTCCACCATTGGCAA
TTCGTATTGCCGCAGCGCTGGGAAGTACACCTGAGTTTTGGCTGCGTCTCCAGAGCGCTTATGACCTGCGTCAGTTGGAA
AATCAAATCGACACGTCTGGGATTGTTTTATATGGCGAGCAGGAAGAAATGTCGCAGGTCACTTCAAAGTATTAA