Detailed information of TA system TA240987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2260549..2261468
• Replicon: chromosome
• Accession: NZ_CP095020
• Organism: Mycobacterium tuberculosis strain SGF0232017

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: MUW65_RS10620
• Protein ID: WP_024753682.1
• Coordinates: 2260863..2261468 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: MUW65_RS10615
• Protein ID: WP_003410124.1
• Coordinates: 2260549..2260854 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW65_RS10580 (MUW65_10580)	2255812..2256012	+	201	Protein_2091	hypothetical protein	-
MUW65_RS10590 (MUW65_10590)	2257368..2257745	+	378	Protein_2093	hypothetical protein	-
MUW65_RS10595 (MUW65_10595)	2257742..2258782	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
MUW65_RS10600 (MUW65_10600)	2259024..2259743	+	720	WP_003410108.1	DUF433 domain-containing protein	-
MUW65_RS10605 (MUW65_10605)	2259733..2260149	+	417	WP_003410114.1	hypothetical protein	-
MUW65_RS10610 (MUW65_10610)	2260165..2260464	-	300	WP_003410120.1	hypothetical protein	-
MUW65_RS10615 (MUW65_10615)	2260549..2260854	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
MUW65_RS10620 (MUW65_10620)	2260863..2261468	-	606	WP_024753682.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MUW65_RS10625 (MUW65_10625)	2261493..2261852	-	360	WP_003410131.1	hypothetical protein	-
MUW65_RS10630 (MUW65_10630)	2262012..2262602	-	591	WP_223695536.1	hypothetical protein	-
MUW65_RS10635 (MUW65_10635)	2262641..2262877	-	237	WP_003410133.1	hypothetical protein	-
MUW65_RS10640 (MUW65_10640)	2263084..2263980	-	897	WP_003410136.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22743.97 Da
• Isoelectric Point: 7.6272

>T240987 WP_024753682.1 NZ_CP095020:c2261468-2260863 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGNEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T240987 NZ_LT903847:2654025-2654276 [Escherichia coli O127:H6]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT240987 WP_003410124.1 NZ_CP095020:c2260854-2260549 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT240987 NZ_LT903847:2654273-2654527 [Escherichia coli O127:H6]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV30