Detailed information of TA system TA240909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 16217..16874
• Replicon: plasmid pB-8919_NDM-1
• Accession: NZ_CP094993
• Organism: Klebsiella pneumoniae strain SCPM-O-B-8919 (12PKP/19c)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: KF986_RS27975
• Protein ID: WP_000270043.1
• Coordinates: 16524..16874 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KF986_RS27970
• Protein ID: WP_000124640.1
• Coordinates: 16217..16519 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF986_RS27930 (KF986_027930)	11256..11843	+	588	Protein_17	hypothetical protein	-
KF986_RS27935 (KF986_027935)	11899..12654	-	756	WP_000194037.1	IS21-like element ISAs29 family helper ATPase IstB	-
KF986_RS27940 (KF986_027940)	12669..14210	-	1542	WP_001274811.1	IS21-like element ISAs29 family transposase	-
KF986_RS27945 (KF986_027945)	14432..14731	+	300	Protein_20	hypothetical protein	-
KF986_RS27950 (KF986_027950)	14757..14948	+	192	WP_001270409.1	hypothetical protein	-
KF986_RS27955 (KF986_027955)	14951..15478	+	528	WP_001210659.1	thermonuclease family protein	-
KF986_RS27960 (KF986_027960)	15536..15808	+	273	WP_001043046.1	HU family DNA-binding protein	-
KF986_RS27965 (KF986_027965)	15897..16190	+	294	WP_001239998.1	hypothetical protein	-
KF986_RS27970 (KF986_027970)	16217..16519	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
KF986_RS27975 (KF986_027975)	16524..16874	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KF986_RS27980 (KF986_027980)	17037..17585	+	549	WP_001061573.1	hypothetical protein	-
KF986_RS27985 (KF986_027985)	17926..18120	+	195	WP_000343597.1	hypothetical protein	-
KF986_RS27990 (KF986_027990)	18131..18502	+	372	WP_000516918.1	hypothetical protein	-
KF986_RS27995 (KF986_027995)	18495..18965	+	471	WP_001281821.1	hypothetical protein	-
KF986_RS28000 (KF986_028000)	18980..19315	-	336	WP_000683477.1	hypothetical protein	-
KF986_RS28005 (KF986_028005)	19412..19900	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
KF986_RS28010 (KF986_028010)	19903..20400	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / armA / msr(E) / mph(E) / dfrA12 / aadA2 / blaNDM-1 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa	htpB	1..174840	174840

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T240909 WP_000270043.1 NZ_CP094993:c16874-16524 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T240909 NZ_LT853887:c17267-16983 [Xanthomonas fragariae]
GTGCCACACCTGATTTGGACGCCGCCGGCACTCGCGGACGTTCAACGCCTGTATCGTTTCCTTGCTCCGAAGGATGCCGA
CGCTGCCCGCCGTGCGGTGCAAGCAATCCGCGCGCAGGTCAAAATCCTGGCGCATCAGCCTCGCATCGGGCGACCCGTCG
AGGACATGGCCCCCGAGTTCCGCGATTGGCTCATCGACTTTGGCGATAGCGGCTACGTGGCCCGGTATCGCATCAACGAA
AACATGGTGACGATCTTGGCCGTTCGGCACCAAAAGGAAGCGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT240909 WP_000124640.1 NZ_CP094993:c16519-16217 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT240909 NZ_LT853887:c17533-17255 [Xanthomonas fragariae]
ATGGCATCGCCAACCTCGCTCAAGCTCGATGACGAGCTGAAAGGGCGTGTGCAACACCTCGCCCAAACCCAGCGCCGCAC
GCCGCACTGGATCATGCGTGAAGCCATTGCGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGACCTTCAAACAAGACGCGC
TCCGCGCTTGGGAGGACTATCAGCGCACCGGCCTGCACCTGACGCTCGAAGAAACCGATGCGTGGCTGGCCAAGCTCGAA
GCTGGCGAGGATGCGGAACTGCCAGCGTGCCACACCTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9