Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3531308..3531849 Replicon chromosome
Accession NC_012947
Organism Escherichia coli BL21-Gold(DE3)pLysS AG

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag ECBD_RS17700 Protein ID WP_000615981.1
Coordinates 3531571..3531849 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag ECBD_RS17695 Protein ID WP_000729704.1
Coordinates 3531308..3531568 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECBD_RS17675 3526991..3527776 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
ECBD_RS17680 3527748..3529460 + 1713 Protein_3375 flagellar biosynthesis protein FlhA -
ECBD_RS17685 3529676..3530173 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
ECBD_RS17690 3530349..3531122 - 774 WP_001353765.1 C40 family peptidase -
ECBD_RS17695 3531308..3531568 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECBD_RS17700 3531571..3531849 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECBD_RS17705 3532005..3532745 + 741 WP_001225679.1 murein L,D-transpeptidase -
ECBD_RS17710 3532716..3533483 - 768 WP_000333379.1 class II glutamine amidotransferase -
ECBD_RS17715 3533689..3534267 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3518837..3557929 39092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T24089 WP_000615981.1 NC_012947:3531571-3531849 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T24089 NC_012947:3531571-3531849 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT24089 WP_000729704.1 NC_012947:3531308-3531568 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT24089 NC_012947:3531308-3531568 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References