Detailed information of TA system TA024088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3525119..3525813
• Replicon: chromosome
• Accession: NC_012947
• Organism: Escherichia coli BL21-Gold(DE3)pLysS AG

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: ECBD_RS17660
• Protein ID: WP_001263493.1
• Coordinates: 3525119..3525517 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ECBD_RS17665
• Protein ID: WP_000554757.1
• Coordinates: 3525520..3525813 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECBD_RS17630	3520119..3521363	-	1245	WP_000189541.1	esterase FrsA	-
-	3520779..3520859	-	81	NuclAT_13	-	-
-	3520779..3520859	-	81	NuclAT_13	-	-
-	3520779..3520859	-	81	NuclAT_13	-	-
-	3520779..3520859	-	81	NuclAT_13	-	-
ECBD_RS17635	3521455..3521913	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ECBD_RS17640	3522174..3523631	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
ECBD_RS17645	3523688..3524209	-	522	Protein_3368	peptide chain release factor H	-
ECBD_RS17650	3524208..3524411	-	204	Protein_3369	RNA ligase RtcB family protein	-
ECBD_RS17655	3524657..3525109	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
ECBD_RS17660	3525119..3525517	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ECBD_RS17665	3525520..3525813	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ECBD_RS17670	3525865..3526920	-	1056	WP_001226164.1	DNA polymerase IV	-
ECBD_RS17675	3526991..3527776	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
ECBD_RS17680	3527748..3529460	+	1713	Protein_3375	flagellar biosynthesis protein FlhA	-
ECBD_RS17685	3529676..3530173	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3518837..3557929	39092

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T24088 WP_001263493.1 NC_012947:c3525517-3525119 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T24088 NC_012947:c3525517-3525119 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT24088 WP_000554757.1 NC_012947:c3525813-3525520 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT24088 NC_012947:c3525813-3525520 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6