Detailed information of TA system TA240872

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2828050..2828275
• Replicon: chromosome
• Accession: NZ_CP094987
• Organism: Shigella flexneri strain 3160_NCHU22

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: MT485_RS14450
• Protein ID: WP_000813254.1
• Coordinates: 2828050..2828205 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2828217..2828275 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT485_RS14395	2823367..2823715	-	349	Protein_2796	IS66 family insertion sequence element accessory protein TnpB	-
MT485_RS14400	2823712..2824384	-	673	Protein_2797	IS66-like element accessory protein TnpA	-
MT485_RS14405	2824483..2824698	-	216	WP_000839572.1	class II holin family protein	-
MT485_RS14430	2825493..2826181	-	689	Protein_2799	bacteriophage antitermination protein Q	-
MT485_RS14435	2826178..2826543	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
MT485_RS14440	2826544..2827602	-	1059	WP_001265248.1	DUF968 domain-containing protein	-
MT485_RS14445	2827604..2827882	-	279	WP_011069426.1	hypothetical protein	-
MT485_RS14450	2828050..2828205	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2828217..2828275	+	59	-	-	Antitoxin
MT485_RS14455	2828857..2829273	-	417	WP_005069274.1	hypothetical protein	-
MT485_RS14460	2829300..2829440	+	141	Protein_2805	DUF4224 domain-containing protein	-
MT485_RS14465	2829440..2830490	+	1051	Protein_2806	tyrosine-type recombinase/integrase	-
MT485_RS14475	2830701..2831498	+	798	WP_001325918.1	DgsA anti-repressor MtfA	-
MT485_RS14485	2831836..2833098	+	1263	Protein_2808	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2795432..2866153	70721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T240872 WP_000813254.1 NZ_CP094987:c2828205-2828050 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T240872 NZ_LT838200:1832515-1832766 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT240872 NZ_CP094987:2828217-2828275 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin