Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1124837..1125681 Replicon chromosome
Accession NZ_CP094987
Organism Shigella flexneri strain 3160_NCHU22

Toxin (Protein)


Gene name itaT Uniprot ID A0A4P7TPN5
Locus tag MT485_RS05450 Protein ID WP_005060796.1
Coordinates 1124837..1125235 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag MT485_RS05460 Protein ID WP_005053053.1
Coordinates 1125370..1125681 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT485_RS05435 (1120005) 1120005..1121735 - 1731 WP_000645013.1 hypothetical protein -
MT485_RS05440 (1121788) 1121788..1123941 - 2154 WP_005060811.1 SEL1-like repeat protein -
MT485_RS05445 (1124092) 1124092..1124789 + 698 WP_223368647.1 IS1 family transposase -
MT485_RS05450 (1124837) 1124837..1125235 - 399 WP_005060796.1 GNAT family N-acetyltransferase Toxin
MT485_RS05455 (1125228) 1125228..1125386 - 159 WP_005053055.1 hypothetical protein -
MT485_RS05460 (1125370) 1125370..1125681 - 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
MT485_RS05465 (1125865) 1125865..1126755 - 891 WP_000971346.1 heme o synthase -
MT485_RS05470 (1126767) 1126767..1127096 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
MT485_RS05475 (1127096) 1127096..1127710 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
MT485_RS05480 (1127700) 1127700..1129691 - 1992 Protein_1056 cytochrome o ubiquinol oxidase subunit I -
MT485_RS05485 (1129713) 1129713..1130210 - 498 Protein_1057 COX aromatic rich motif-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1121788..1132177 10389
flank IS/Tn - - 1124286..1124789 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(52-112)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14335.69 Da        Isoelectric Point: 9.1674

>T240858 WP_005060796.1 NZ_CP094987:c1125235-1124837 [Shigella flexneri]
VRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKK
IHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 399 bp

>T240858 NZ_LT838196:3539923-3540141 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT240858 WP_005053053.1 NZ_CP094987:c1125681-1125370 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT240858 NZ_LT838196:3539523-3539897 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPN5

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References