Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1044888..1045582 Replicon chromosome
Accession NZ_CP094987
Organism Shigella flexneri strain 3160_NCHU22

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag MT485_RS05050 Protein ID WP_001263491.1
Coordinates 1045184..1045582 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag MT485_RS05045 Protein ID WP_000554759.1
Coordinates 1044888..1045181 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT485_RS05025 (1040527) 1040527..1041024 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
MT485_RS05030 (1041241) 1041241..1042953 - 1713 Protein_967 flagellar biosynthesis protein FlhA -
MT485_RS05035 (1042925) 1042925..1043710 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
MT485_RS05040 (1043781) 1043781..1044836 + 1056 WP_001226172.1 DNA polymerase IV -
MT485_RS05045 (1044888) 1044888..1045181 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MT485_RS05050 (1045184) 1045184..1045582 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MT485_RS05055 (1045592) 1045592..1046044 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
MT485_RS05060 (1046290) 1046290..1047333 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
MT485_RS05065 (1047449) 1047449..1048009 + 561 Protein_974 peptide chain release factor H -
MT485_RS05070 (1048066) 1048066..1049523 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
MT485_RS05075 (1049785) 1049785..1050243 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T240857 WP_001263491.1 NZ_CP094987:1045184-1045582 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T240857 NZ_LT838196:2736983-2737090 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT240857 WP_000554759.1 NZ_CP094987:1044888-1045181 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT240857 NZ_LT838196:c2736936-2736870 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References